HCLS1 水平升高与心力衰竭的相关性：一种诊断生物标志物。
Correlation between elevated HCLS1 levels and heart failure: A diagnostic biomarker.
机构信息
Clinical Lab Center, Beijing Luhe Hospital, Capital Medical University, Beijing, China.
Blood Transfusion Department, Beijing Luhe Hospital, Capital Medical University, Beijing, China.
出版信息
Medicine (Baltimore). 2024 Jun 7;103(23):e38484. doi: 10.1097/MD.0000000000038484.
The correlation between hematopoietic cell-specific lyn substrate 1 (HCLS1) expression levels and heart failure (HF) remains unclear. HF datasets GSE192886 and GSE196656 profiles were generated from GPL24676 and GPL20301 platforms in gene expression omnibus (GEO) database and differentially expressed genes (DEGs) were obtained, which was followed by weighted gene co-expression network analysis, protein-protein interaction (PPI) networks, functional enrichment analysis and comparative toxicogenomics database (CTD) analysis. Heatmaps of gene expression levels were plotted. TargetScan was used to screen miRNAs regulating central DEGs. A total of 500 DEGs were found and mainly concentrated in leukocyte activation, protein phosphorylation, and protein complexes involved in cell adhesion, PI3K Akt signaling pathway, Notch signaling pathway, and right ventricular cardiomyopathy. PPI network identified 15 core genes (HCLS1, FERMT3, CD53, CD34, ITGAL, EP300, LYN, VAV1, ITGAX, LEP, ITGB1, IGF1, MMP9, SMAD2, RAC2). Heatmap shows that 4 genes (EP300, CD53, HCLS1, LYN) are highly expressed in HF tissue samples. We found that 4 genes (EP300, CD53, HCLS1, LYN) were associated with heart diseases, cardiovascular diseases, edema, rheumatoid arthritis, necrosis, and inflammation. HCLS1 is highly expressed in HF and maybe its target.
造血细胞特异性 Lyn 底物 1（HCLS1）表达水平与心力衰竭（HF）之间的相关性尚不清楚。HF 数据集 GSE192886 和 GSE196656 谱图来自基因表达综合数据库（GEO）数据库中的 GPL24676 和 GPL20301 平台，获得差异表达基因（DEGs），随后进行加权基因共表达网络分析、蛋白质-蛋白质相互作用（PPI）网络、功能富集分析和比较毒理基因组学数据库（CTD）分析。绘制基因表达水平的热图。使用 TargetScan 筛选调节中心 DEGs 的 miRNA。共发现 500 个 DEGs，主要集中在白细胞活化、蛋白磷酸化以及涉及细胞黏附、PI3K Akt 信号通路、Notch 信号通路和右心室心肌病的蛋白复合物。PPI 网络确定了 15 个核心基因（HCLS1、FERMT3、CD53、CD34、ITGAL、EP300、LYN、VAV1、ITGAX、LEP、ITGB1、IGF1、MMP9、SMAD2、RAC2）。热图显示 4 个基因（EP300、CD53、HCLS1、LYN）在 HF 组织样本中高表达。我们发现 4 个基因（EP300、CD53、HCLS1、LYN）与心脏病、心血管疾病、水肿、类风湿性关节炎、坏死和炎症有关。HCLS1 在 HF 中高表达，可能是其靶点。
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