School of Medicine, Chung Shan Medical University, Taichung, Taiwan.
Department of Urology, Chung Shan Medical University Hospital, Taichung, Taiwan.
PLoS One. 2024 Jun 7;19(6):e0303334. doi: 10.1371/journal.pone.0303334. eCollection 2024.
Exercise offers numerous benefits to cancer patients and plays an essential role in postsurgical cancer rehabilitation. However, there is a lack of research examining the effects of exercise after the surgical stress of nephrectomy. To address this gap, we created an animal model that simulated patients who had undergone nephrectomy with or without an exercise intervention. Next, we performed a bioinformatic analysis based on the data generated by the RNA sequencing of the lung tissue sample. An overrepresentation analysis was conducted using two genome databases (Gene Ontology and Kyoto Encyclopedia of Genes and Genomes [KEGG]). A KEGG analysis of the exercise-treated nephrectomy mice revealed enrichment in immune-related pathways, particularly in the NF-κB and B cell-related pathways. The expression of CD79A and IGHD, which are responsible for B cell differentiation and proliferation, was upregulated in the nephrectomy mice. Differential gene expression was categorized as significantly upregulated or downregulated according to nephrectomy and exercise groups. Notably, we identified several gene expression reversals in the nephrectomy groups with exercise that were not found in the nephrectomy without exercise or control groups. Our preliminary results potentially reveal a genetic landscape for the underlying mechanisms of the effects of exercise on our nephrectomy model.
锻炼对癌症患者有诸多益处，并在癌症术后康复中发挥着重要作用。然而，目前缺乏研究来检验肾切除术后锻炼对患者的影响。为了填补这一空白，我们构建了一个动物模型，模拟接受或未接受肾切除手术及锻炼干预的患者。然后，我们对肺组织样本的 RNA 测序数据进行了生物信息学分析。我们使用两个基因组数据库（基因本体论和京都基因与基因组百科全书[KEGG]）对数据进行了过表达分析。KEGG 分析显示，接受锻炼的肾切除小鼠中与免疫相关的通路富集，特别是 NF-κB 和 B 细胞相关通路。负责 B 细胞分化和增殖的 CD79A 和 IGHD 的表达在肾切除小鼠中上调。根据肾切除和锻炼组，将差异基因表达分类为显著上调或下调。值得注意的是，我们在接受锻炼的肾切除组中发现了一些在未接受锻炼的肾切除组或对照组中未发现的基因表达逆转。我们的初步结果可能揭示了锻炼对我们的肾切除模型影响的潜在遗传机制。
