Dong Zixuan, Han Weiju, Jiang Panyu, Hao Lijing, Fu Xiaoling
The Second Affiliated Hospital, School of Biomedical Sciences and Engineering, South China University of Technology, Guangzhou 511442, China.
National Engineering Research Center for Tissue Restoration and Reconstruction and Innovation Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.
Regen Biomater. 2024 May 7;11:rbae052. doi: 10.1093/rb/rbae052. eCollection 2024.
Mitochondrial network architecture, which is closely related to mitochondrial function, is mechanically sensitive and regulated by multiple stimuli. However, the effects of microtopographic cues on mitochondria remain poorly defined. Herein, polycaprolactone (PCL) surfaces were used as models to investigate how micropatterns regulate mitochondrial network architecture and function in rat adipose-derived stem cells (rASCs). It was found that large pit (LP)-induced rASCs to form larger and more complex mitochondrial networks. Consistently, the expression of key genes related to mitochondrial dynamics revealed that mitochondrial fusion (MFN1 and MFN2) and midzone fission (DRP1 and MFF) were increased in rASCs on LP. In contrast, the middle pit (MP)-enhanced mitochondrial biogenesis, as evidenced by the larger mitochondrial area and higher expression of PGC-1. Both LP and MP promoted ATP production in rASCs. It is likely that LP increased ATP levels through modulating mitochondrial network architecture while MP stimulated mitochondria biogenesis to do so. Our study clarified the regulation of micropatterned surfaces on mitochondria, highlighting the potential of LP and MP as a simple platform to stimulate mitochondria and the subsequent cellular function of MSCs.
线粒体网络结构与线粒体功能密切相关，具有机械敏感性并受多种刺激调控。然而，微观形貌线索对线粒体的影响仍不清楚。在此，聚己内酯（PCL）表面被用作模型，以研究微图案如何调节大鼠脂肪来源干细胞（rASCs）中的线粒体网络结构和功能。研究发现，大凹坑（LP）诱导rASCs形成更大且更复杂的线粒体网络。一致地，与线粒体动力学相关的关键基因表达显示，LP上的rASCs中线粒体融合（MFN1和MFN2）和中间区裂变（DRP1和MFF）增加。相比之下，中凹坑（MP）增强了线粒体生物合成，较大的线粒体面积和较高的PGC-1表达证明了这一点。LP和MP均促进了rASCs中的ATP产生。LP可能通过调节线粒体网络结构增加ATP水平，而MP则通过刺激线粒体生物合成来实现。我们的研究阐明了微图案表面对线粒体的调控，突出了LP和MP作为刺激线粒体及随后间充质干细胞细胞功能的简单平台的潜力。
