Xie Yifei, Hou Zhiying, Shi Miao, Wang Qiaoyan, Yang Zhengfu, Lim Kean-Jin, Wang Zhengjia
State Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, China.
Plants (Basel). 2023 Mar 20;12(6):1378. doi: 10.3390/plants12061378.
Pecan () nuts are delicious and rich in unsaturated fatty acids, which are beneficial for human health. Their yield is closely related to several factors, such as the ratio of female and male flowers. We sampled and paraffin-sectioned female and male flower buds for one year and determined the stages of initial flower bud differentiation, floral primordium formation, and pistil and stamen primordium formation. We then performed transcriptome sequencing on these stages. Our data analysis suggested that FLOWERING LOCUS T (FT) and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 play a role in flower bud differentiation. was highly expressed in the early stage of female flower buds and may play a role in regulating flower bud differentiation and flowering time. Genes such as and were expressed during male flower bud development. belongs to the NF-Y transcription factor family and may initiate downstream events leading to floral transformation. promoted the transformation of leaf buds to flower buds. may have been involved in the establishment of floral meristem characteristics and the determination of floral organ characteristics. Our results lay a foundation for the control and subsequent regulation of female and male flower bud differentiation and yield improvement.
山核桃坚果美味可口，富含不饱和脂肪酸，对人体健康有益。其产量与多个因素密切相关，如雌花和雄花的比例。我们对雌花和雄花的芽进行了为期一年的采样和石蜡切片，并确定了花芽分化初期、花原基形成以及雌蕊和雄蕊原基形成的阶段。然后我们对这些阶段进行了转录组测序。我们的数据分析表明，开花位点T（FT）和CONSTANS过表达抑制因子1在花芽分化中起作用。在雌花芽的早期高度表达，可能在调节花芽分化和开花时间方面发挥作用。诸如和等基因在雄花芽发育过程中表达。属于NF-Y转录因子家族，可能启动导致花转变的下游事件。促进了叶芽向花芽的转变。可能参与了花分生组织特征的建立和花器官特征的确定。我们的研究结果为控制和随后调节雌花和雄花芽分化以及提高产量奠定了基础。
