Gripenberg M, Helve T
Rheumatol Int. 1986;6(2):53-5. doi: 10.1007/BF00541504.
Sera obtained from 53 patients with systemic lupus erythematosus (SLE) were investigated for the presence of immunoglobulin class-specific antibodies against native (ds)DNA and denatured (ss)DNA. The methods employed were the Crithidia luciliae test and an enzyme-linked immunosorbent assay (ELISA), respectively. Anti-dsDNA antibodies of IgG class were seen in 42%, IgM-anti-dsDNA antibodies in 43%, and IgA-anti-dsDNA antibodies in 30% of the patients. There was an association between the presence of both IgG- and IgA anti-dsDNA antibodies and the activity of the disease. Patients with active nephritis also had anti-dsDNA antibodies of IgG and IgA class significantly more often than patients with inactive nephritis or without renal disease. IgG-anti-ssDNA antibodies were seen in 89%, IgM-anti-ssDNA antibodies in 51%, and IgA-anti-ssDNA antibodies in 66% of the patients. Patients with nephritis had low levels of antibodies to ssDNA of IgM class. We suggest that immunoglobulin class-specific anti-DNA antibodies should be determined in the diagnosis and monitoring of SLE.
对53例系统性红斑狼疮（SLE）患者的血清进行检测，以确定是否存在针对天然（双链）DNA和变性（单链）DNA的免疫球蛋白类别特异性抗体。所采用的方法分别是利什曼原虫试验和酶联免疫吸附测定（ELISA）。42%的患者出现IgG类抗双链DNA抗体，43%的患者出现IgM类抗双链DNA抗体，30%的患者出现IgA类抗双链DNA抗体。IgG和IgA抗双链DNA抗体的存在与疾病活动度之间存在关联。活动性肾炎患者出现IgG和IgA类抗双链DNA抗体的频率也明显高于非活动性肾炎患者或无肾脏疾病的患者。89%的患者出现IgG类抗单链DNA抗体，51%的患者出现IgM类抗单链DNA抗体，66%的患者出现IgA类抗单链DNA抗体。肾炎患者的IgM类抗单链DNA抗体水平较低。我们建议在SLE的诊断和监测中应测定免疫球蛋白类别特异性抗DNA抗体。
