一种用于定量血清铁蛋白的生物素-抗生物素蛋白酶免疫测定法。

A biotin-avidin enzyme immunoassay for the quantitation of serum ferritin.

作者信息

Lee M, Chang J, Carlson D, Burgett M

出版信息

Clin Chim Acta. 1985 Apr 15;147(2):109-16. doi: 10.1016/0009-8981(85)90071-3.

Abstract

A biotin-avidin enzyme immunoassay (EIA) for measurement of ferritin in human serum is described. This procedure involves the use of specific antibody immobilized on beads, biotin-labeled specific antibody and enzyme-labeled avidin. Reproducible results were achieved within 2 h for ferritin in serum in the range of 5-500 ng/ml. Ferritin levels as low as 0.1 ng/ml can be measured. The biotin-avidin EIA, a radioimmunoassay kit, and an EIA kit were used to determine serum ferritin levels in healthy adults. Good agreement was found between the biotin-avidin EIA and the other methods.

摘要

本文描述了一种用于测定人血清中铁蛋白的生物素-抗生物素蛋白酶免疫测定法（EIA）。该方法使用固定在珠子上的特异性抗体、生物素标记的特异性抗体和酶标记的抗生物素蛋白。对于血清中浓度在5 - 500 ng/ml范围内的铁蛋白，可在2小时内获得可重复的结果。最低可检测到0.1 ng/ml的铁蛋白水平。使用生物素-抗生物素蛋白EIA、放射免疫测定试剂盒和EIA试剂盒来测定健康成年人的血清铁蛋白水平。发现生物素-抗生物素蛋白EIA与其他方法之间具有良好的一致性。