Wood W G
J Clin Chem Clin Biochem. 1981 Sep;19(9):947-52. doi: 10.1515/cclm.1981.19.9.947.
Eleven commercial kits for serum ferritin have been compared. Seven kits used RIA and four IRMA methodology. Seven kits used human liver ferritin, two human spleen ferritin and two undefined human ferritin as standard material. Incubation times varied between 30 minutes and 36 hours. Only four kits gave normal ranges determined with the kit in question. All values were compared with the kit in routine use. Although correlation of results was good in 10 cases, (r = 0.903-0.993) the numerical values varied greatly, (slope of the regression line b = 0.437-1.94). These values were obtained from at least 30 sera for each kit. Seven kits gave significantly different results from the routine kit (p less than 0.05 -- Wilcoxon matched pairs test -- two tail value). Inter and Intra-assay coefficients of variation were under 12.5% in all cases within the range of interest. Four kits showed a pronounced non-linear correlation with the routine kit, and one of the IRMA kits exhibited a high-dose hook effect within the standard curve. The other three IRMA kits showed no such effect up to 2000 micrograms . liter-1 (under assay conditions).
对11种血清铁蛋白商业试剂盒进行了比较。7种试剂盒采用放射免疫分析（RIA）法，4种采用免疫放射分析（IRMA）法。7种试剂盒用人肝铁蛋白作为标准物质，2种用人脾铁蛋白，2种用未明确的人铁蛋白作为标准物质。孵育时间在30分钟至36小时之间。只有4种试剂盒给出了用该试剂盒测定的正常范围。所有数值均与常规使用的试剂盒进行比较。尽管10种情况下结果的相关性良好（r = 0.903 - 0.993），但数值差异很大（回归线斜率b = 0.437 - 1.94）。这些数值是每种试剂盒至少从30份血清中获得的。7种试剂盒与常规试剂盒的结果有显著差异（p < 0.05——Wilcoxon配对检验——双侧值）。在感兴趣的范围内，所有情况下批内和批间变异系数均低于12.5%。4种试剂盒与常规试剂盒呈现明显的非线性相关性，其中一种IRMA试剂盒在标准曲线内表现出高剂量钩效应。其他三种IRMA试剂盒在2000微克·升-1以下（在测定条件下）未显示出这种效应。
