从人肠道中分离得到的链霉菌 HFM-2 的益生菌潜力及其对病原菌的抗生物膜特性。

Probiotic potential of Streptomyces levis strain HFM-2 isolated from human gut and its antibiofilm properties against pathogenic bacteria.

机构信息

Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab, India.

出版信息

BMC Microbiol. 2024 Jun 11;24(1):208. doi: 10.1186/s12866-024-03353-x.

DOI:10.1186/s12866-024-03353-x

PMID:38862894

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11165917/

Abstract

BACKGROUND

Antimicrobial resistance (AMR) is a serious worldwide public health concern that needs immediate action. Probiotics could be a promising alternative for fighting antibiotic resistance, displaying beneficial effects to the host by combating diseases, improving growth, and stimulating the host immune responses against infection. This study was conducted to evaluate the probiotic, antibacterial, and antibiofilm potential of Streptomyces levis strain HFM-2 isolated from the healthy human gut.

RESULTS

In vitro antibacterial activity in the cell-free supernatant of S. levis strain HFM-2 was evaluated against different pathogens viz. K. pneumoniae sub sp. pneumoniae, S. aureus, B. subtilis, VRE, S. typhi, S. epidermidis, MRSA, V. cholerae, M. smegmatis, E. coli, P. aeruginosa and E. aerogenes. Further, the ethyl acetate extract from S. levis strain HFM-2 showed strong biofilm inhibition against S. typhi, K. pneumoniae sub sp. pneumoniae, P. aeruginosa and E. coli. Fluorescence microscopy was used to detect biofilm inhibition properties. MIC and MBC values of EtOAc extract were determined at 500 and 1000 µg/mL, respectively. Further, strain HFM-2 showed high tolerance in gastric juice, pancreatin, bile, and at low pH. It exhibited efficient adhesion properties, displaying auto-aggregation (97.0%), hydrophobicity (95.71%, 88.96%, and 81.15% for ethyl acetate, chloroform and xylene, respectively), and showed 89.75%, 86.53%, 83.06% and 76.13% co-aggregation with S. typhi, MRSA, S. pyogenes and E. coli, respectively after 60 min of incubation. The S. levis strain HFM-2 was susceptible to different antibiotics such as tetracycline, streptomycin, kanamycin, ciprofloxacin, erythromycin, linezolid, meropenem, amikacin, gentamycin, clindamycin, moxifloxacin and vancomycin, but resistant to ampicillin and penicillin G.

CONCLUSION

The study shows that S. levis strain HFM-2 has significant probiotic properties such as good viability in bile, gastric juice, pancreatin environment, and at low pH; proficient adhesion properties, and antibiotic susceptibility. Further, the EtOAc extract of Streptomyces levis strain HFM-2 has a potent antibiofilm and antibacterial activity against antibacterial-resistant clinical pathogens.

摘要

背景

抗菌药物耐药性（AMR）是一个严重的全球公共卫生问题，需要立即采取行动。益生菌可能是对抗抗生素耐药性的一种有前途的选择，通过对抗疾病、促进生长和刺激宿主对感染的免疫反应，对宿主产生有益的影响。本研究旨在评估从健康人体肠道中分离出的解淀粉芽孢杆菌 HFM-2 的益生菌、抗菌和抗生物膜潜力。

结果

评估了解淀粉芽孢杆菌 HFM-2 细胞游离上清液对不同病原体的体外抗菌活性，如肺炎克雷伯菌亚种肺炎、金黄色葡萄球菌、枯草芽孢杆菌、万古霉素耐药肠球菌、伤寒沙门氏菌、表皮葡萄球菌、耐甲氧西林金黄色葡萄球菌、霍乱弧菌、耻垢分枝杆菌、大肠杆菌、铜绿假单胞菌和阴沟肠杆菌。此外，解淀粉芽孢杆菌 HFM-2 的乙酸乙酯提取物对伤寒沙门氏菌、肺炎克雷伯菌亚种肺炎、铜绿假单胞菌和大肠杆菌具有很强的生物膜抑制作用。通过荧光显微镜检测生物膜抑制特性。乙酸乙酯提取物的 MIC 和 MBC 值分别为 500 和 1000 μg/mL。此外，菌株 HFM-2 在胃液、胰酶、胆汁和低 pH 值条件下具有较高的耐受性。它表现出高效的粘附特性，显示出自我聚集（97.0%）、疏水性（乙酸乙酯、氯仿和二甲苯分别为 95.71%、88.96%和 81.15%），并在 60 分钟孵育后与伤寒沙门氏菌、耐甲氧西林金黄色葡萄球菌、化脓性链球菌和大肠杆菌分别显示 89.75%、86.53%、83.06%和 76.13%的共聚集。解淀粉芽孢杆菌 HFM-2 对不同抗生素如四环素、链霉素、卡那霉素、环丙沙星、红霉素、利奈唑胺、美罗培南、阿米卡星、庆大霉素、克林霉素、莫西沙星和万古霉素敏感，但对氨苄西林和青霉素 G 耐药。