Chávez-Hernández Michelle, Ortiz-Álvarez Jossue, Morales-Jiménez Jesús, Villa-Tanaca Lourdes, Hernández-Rodríguez César
Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prol. de Carpio y Plan de Ayala, Col. Sto. Tomás s/n, Ciudad de México 11340, Mexico.
Programa "Investigadoras e Investigadores por México". Consejo Nacional de Humanidades, Ciencias y Tecnologías (CONAHCYT). Av. de los Insurgentes Sur 1582, Crédito Constructor, Benito Juárez, Ciudad de México 03940, Mexico.
Microorganisms. 2023 Oct 13;11(10):2551. doi: 10.3390/microorganisms11102551.
The increasing number of infections caused by antimicrobial multi-resistant microorganisms has led to the search for new microorganisms capable of producing novel antibiotics. This work proposes sp. nov. as a new member of the Streptomycetaceae family. The strain ENCB-J15 was isolated from the jungle soil in Palenque National Park, Chiapas, Mexico. The strain formed pale brown, dry, tough, and buried colonies in the agar with no diffusible pigment in GAE (glucose-asparagine-yeast extract) medium. Scanning electron micrographs showed typical mycelium with long chains of smooth and oval-shaped spores (3-10 m). The strain grew in all of the International Project (ISP)'s media at 28-37 °C with a pH of 6-9 and 0-10% NaCl. ENCB-J15 assimilated diverse carbon as well as organic and inorganic nitrogen sources. The strain also exhibited significant inhibitory activity against the prodigiosin synthesis of and the inhibition of the formation and destruction of biofilms of ESKAPE strains of and . The draft genome sequencing of ENCB-J15 revealed a 7.6 Mb genome with a high G + C content (71.6%), 6833 total genes, and 6746 genes encoding putative proteins. A total of 26 accessory clusters of proteins associated with carbon sources and amino acid catabolism, DNA modification, and the antibiotic biosynthetic process were annotated. The 16S rRNA gene phylogeny, core-proteome phylogenomic tree, and virtual genome fingerprints support that ENCB-J15 is a new species related to and . Similarly, its average nucleotide identity (ANI) (96.4%), average amino acid identity (AAI) (96.06%), and virtual DNA-DNA hybridization (67.3%) provide evidence to recognize it as a new species. Comparative genomics revealed that and its closest related species maintain a well-conserved genomic synteny. This work proposes sp. nov. as a novel species that expresses anti-biofilm and anti-quorum sensing activities.
由多重耐药微生物引起的感染数量不断增加，促使人们寻找能够产生新型抗生素的新微生物。这项研究提出[具体菌名] sp. nov. 作为链霉菌科的一个新成员。菌株ENCB-J15是从墨西哥恰帕斯州帕伦克国家公园的丛林土壤中分离出来的。该菌株在GAE（葡萄糖-天冬酰胺-酵母提取物）培养基的琼脂中形成浅棕色、干燥、坚韧且埋于培养基中的菌落，无扩散性色素。扫描电子显微镜图像显示出典型的菌丝体，带有长链状光滑且椭圆形的孢子（3 - 10μm）。该菌株在国际链霉菌计划（ISP）的所有培养基中于28 - 37°C、pH值为6 - 9且NaCl浓度为0 - 10%的条件下生长。ENCB-J15能利用多种碳源以及有机和无机氮源。该菌株还对[具体菌名]的灵菌红素合成表现出显著抑制活性，并对[具体菌名]的ESKAPE菌株生物膜的形成和破坏具有抑制作用。ENCB-J15的基因组草图测序显示其基因组大小为7.6 Mb，G + C含量高（71.6%），共有6833个基因，其中6746个基因编码推定蛋白。总共注释了26个与碳源和氨基酸分解代谢、DNA修饰以及抗生素生物合成过程相关的辅助蛋白簇。16S rRNA基因系统发育、核心蛋白质组系统发育树和虚拟基因组指纹图谱均支持ENCB-J15是与[相关菌名]相关的一个新物种。同样，其平均核苷酸同一性（ANI）（96.4%）、平均氨基酸同一性（AAI）（96.06%）和虚拟DNA - DNA杂交（67.3%）为将其识别为一个新物种提供了证据。比较基因组学表明[该菌]与其最密切相关的物种保持着高度保守的基因组共线性。这项研究提出[具体菌名] sp. nov. 作为一种具有抗生物膜和抗群体感应活性的新物种。
