Anhui Province Key Laboratory of Medical Physics and Technology, Institute of Health and Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, Anhui, China.
University of Science and Technology of China, Hefei 230026, Anhui, China.
J Proteome Res. 2024 Jul 5;23(7):2552-2560. doi: 10.1021/acs.jproteome.4c00270. Epub 2024 Jun 12.
Detection of exhaled volatile organic compounds (VOCs) is promising for noninvasive screening of esophageal cancer (EC). Cellular VOC analysis can be used to investigate potential biomarkers. Considering the crucial role of methionine (Met) during cancer development, exploring associated abnormal metabolic phenotypes becomes imperative. In this work, we employed headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) to investigate the volatile metabolic profiles of EC cells (KYSE150) and normal esophageal epithelial cells (HEECs) under a Met regulation strategy. Using untargeted approaches, we analyzed the metabolic VOCs of the two cell types and explored the differential VOCs between them. Subsequently, we utilized targeted approaches to analyze the differential VOCs in both cell types under gradient Met culture conditions. The results revealed that there were five/six differential VOCs between cells under Met-containing/Met-free culture conditions. And the difference in levels of two characteristic VOCs (1-butanol and ethyl 2-methylbutyrate) between the two cell types intensified with the increase of the Met concentration. Notably, this is the first report on VOC analysis of EC cells and the first to consider the effect of Met on volatile metabolic profiles. The present work indicates that EC cells can be distinguished through VOCs induced by Met regulation, which holds promise for providing novel insights into diagnostic strategies.
检测呼出气挥发性有机化合物 (VOCs) 是用于非侵入性筛查食管癌 (EC) 的有前途的方法。细胞 VOC 分析可用于研究潜在的生物标志物。考虑到蛋氨酸 (Met) 在癌症发展过程中的关键作用，探索相关的异常代谢表型变得至关重要。在这项工作中，我们采用顶空固相微萃取-气相色谱-质谱联用 (HS-SPME-GC-MS) 研究了蛋氨酸调控策略下 EC 细胞 (KYSE150) 和正常食管上皮细胞 (HEECs) 的挥发性代谢谱。我们采用无靶向方法分析了两种细胞类型的代谢 VOC，并探讨了它们之间的差异 VOC。随后，我们采用靶向方法分析了梯度 Met 培养条件下两种细胞类型的差异 VOC。结果表明，在含 Met/无 Met 培养条件下，细胞之间有五个/六个差异 VOC。两种特征 VOC（1-丁醇和乙基 2-甲基丁酸酯）在两种细胞类型之间的水平差异随着 Met 浓度的增加而加剧。值得注意的是，这是关于 EC 细胞 VOC 分析的首次报道，也是首次考虑 Met 对挥发性代谢谱的影响。本工作表明，通过 Met 调控诱导的 VOC 可以区分 EC 细胞，这为诊断策略提供了新的见解。
