Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
Poult Sci. 2024 Aug;103(8):103897. doi: 10.1016/j.psj.2024.103897. Epub 2024 May 23.
Campylobacter jejuni continues to be a major public health issue worldwide. Poultry are recognized as the main reservoir for this foodborne pathogen. Implementing measures to decrease C. jejuni colonization on farms has been regarded as the most effective strategy to control the incidence of campylobacteriosis. The probiotics supplementation has been regarded as an attractive approach against C. jejuni in chickens. Here the inhibitory effects of one probiotic B. velezensis isolate CAU277 against C. jejuni was evaluated in vitro and in vivo. The in vitro antimicrobial activity showed that the supernatant of B. velezensis exhibited the most pronounced inhibitory effects on Campylobacter strains compared to other bacterial species. When co-cultured with B. velezensis, the growth of C. jejuni reduced significantly from 7.46 log CFU/mL (24 h) to 1.02 log CFU/mL (48 h). Further, the antimicrobial activity of B. velezensis against C. jejuni remained stable under a broad range of temperature, pH, and protease treatments. The in vivo experiments demonstrated that oral administration of B. velezensis significantly reduced the colonization of C. jejuni by 2.0 log CFU/g of feces in chicken cecum at 15 d postinoculation. In addition, the supplementary of B. velezensis significantly increased microbial species richness and diversity in chicken ileum, especially enhanced the bacterial population of Alistipes and Christensenellaceae, and decreased the existence of Lachnoclostridium. Our study presents that B. velezensis possesses antimicrobial activities against C. jejuni and promotes microbiota diversity in chicken intestines. These findings indicate a potential to develop an effective probiotic additive to control C. jejuni infection in chicken.
空肠弯曲菌仍然是全球主要的公共卫生问题。家禽被认为是这种食源性病原体的主要宿主。在农场减少空肠弯曲菌定植的措施被认为是控制弯曲杆菌病发病率的最有效策略。益生菌的补充被认为是控制鸡中空肠弯曲菌的一种有吸引力的方法。在这里，我们评估了一株益生菌 B. velezensis 分离株 CAU277 对空肠弯曲菌的体外和体内抑制作用。体外抗菌活性表明，与其他细菌种类相比，B. velezensis 的上清液对弯曲杆菌菌株表现出最显著的抑制作用。当与 B. velezensis 共培养时，空肠弯曲菌的生长从 7.46 log CFU/mL（24 h）显著减少到 1.02 log CFU/mL（48 h）。此外，B. velezensis 对空肠弯曲菌的抗菌活性在广泛的温度、pH 和蛋白酶处理下保持稳定。体内实验表明，口服 B. velezensis 可显著降低鸡盲肠中 C. jejuni 的定植，在接种后 15 天粪便中 C. jejuni 的定植减少了 2.0 log CFU/g。此外，B. velezensis 的补充显著增加了鸡回肠中的微生物物种丰富度和多样性，特别是增强了 Alistipes 和 Christensenellaceae 的细菌种群，降低了 Lachnoclostridium 的存在。我们的研究表明，B. velezensis 对 C. jejuni 具有抗菌活性，并促进了鸡肠道微生物多样性。这些发现表明，有可能开发一种有效的益生菌添加剂来控制鸡中空肠弯曲菌感染。
