Clin Lab. 2024 Jun 1;70(6). doi: 10.7754/Clin.Lab.2024.231230.
Heterophilic antibodies (HA) are one of the main substances that interfere with immunology, especially chemiluminescence immunoassay. Non-specific binding, labeling antibodies, bridging to capture antibodies, or labeling antigens can interfere with the detection process, leading to serious discrepancies between the measured results and clinical manifestations, and even delaying clinical diagnosis and treatment.
This paper is a case of epidemic hemorrhagic fever causing pseudo CEA elevation caused by heterophagy induced antibodies in the body.
The patient's CEA detected on the ABBOTT detection platform was 51.1 ng/mL, and on the ROCHE detection platforms it was 4.66 ng/mL, and treated by PEG precipitation it was 45.2 ng/mL, after diluting the sample the CEA was 50.2 ng/mL, meanwhile the patient's platelets were 96 x 109/L and serum creatinine was 188.4 μmol/L, epidemic hemorrhagic fever IgM antibody was positive.
When the test results do not match clinical symptoms, further confirmation is required through additional testing. Patients who use mouse monoclonal antibody preparations for diagnosis or treatment may have human anti-mouse antibodies in their serum, and the test results may falsely increase or decrease.
异嗜性抗体（HA）是干扰免疫学检测的主要物质之一，尤其在化学发光免疫分析中。非特异性结合、标记抗体、桥联到捕获抗体或标记抗原都可能干扰检测过程，导致测量结果与临床表现严重不符，甚至延误临床诊断和治疗。
本文报道了一例因体内异嗜性抗体引起的流行性出血热导致假性 CEA 升高的病例。
在 ABBOTT 检测平台上，患者的 CEA 检测值为 51.1ng/ml，在 ROCHE 检测平台上为 4.66ng/ml，PEG 沉淀处理后为 45.2ng/ml，稀释样本后 CEA 为 50.2ng/ml，同时患者的血小板为 96×109/L，血清肌酐为 188.4μmol/L，流行性出血热 IgM 抗体阳性。
当检测结果与临床症状不相符时，需要通过进一步的检测进行确认。用于诊断或治疗的鼠源性单克隆抗体制剂的患者，其血清中可能存在人抗鼠抗体，检测结果可能会出现假性升高或降低。
