Freidlin P J, Bock R, Bernath A, Hoexter S, Ziv G, Fromer A, Perlstein Z, Tomer S, Samberg Y
J Vet Pharmacol Ther. 1985 Mar;8(1):105-9. doi: 10.1111/j.1365-2885.1985.tb00931.x.
The minimal inhibitory concentrations (MIC) of apramycin, a unique aminocyclitol antibiotic, for 100 Escherichia coli isolates recovered from clinical cases of avian colibacillosis were determined using the agar dilution method. All isolates were inhibited at apramycin concentration of 8.0 micrograms/ml; 90 and 50% of the isolates were inhibited at 6.6 and 3.4 micrograms/ml, respectively. A commercial injectable product containing 200 mg apramycin/ml was administered intramuscularly (i.m.) to groups of 6- and 12-week-old turkeys at 10, 15 and 20 mg/kg. Apramycin was quickly absorbed from the i.m. injection site. Mean peak serum drug concentrations were reached 1 h after treatment and were 19.5, 27.5 and 36.0 micrograms/ml, respectively. The serum elimination half-life (t 1/2) of the drug ranged between 1.75 h for the 10 mg/kg dose and 2.5 h for the 20 mg/kg dose. Very low concentrations of the drug were found 24 h after treatment. Duration of serum apramycin concentrations in relation to the MIC, dose, and age of birds was determined.
采用琼脂稀释法测定了独特的氨基环醇类抗生素阿普拉霉素对从禽大肠杆菌病临床病例中分离出的100株大肠杆菌的最低抑菌浓度（MIC）。所有分离株在阿普拉霉素浓度为8.0微克/毫升时均受到抑制；分别有90%和50%的分离株在6.6微克/毫升和3.4微克/毫升时受到抑制。将一种每毫升含200毫克阿普拉霉素的市售注射用产品以10、15和20毫克/千克的剂量分别肌肉注射给6周龄和12周龄的火鸡组。阿普拉霉素从肌肉注射部位迅速吸收。给药后1小时达到平均血清药物峰值浓度，分别为19.5、27.5和36.0微克/毫升。该药物的血清消除半衰期（t 1/2）在10毫克/千克剂量时为1.75小时，在20毫克/千克剂量时为2.5小时。给药后24小时发现药物浓度非常低。测定了血清阿普拉霉素浓度与MIC、剂量和禽龄的关系。
