Department of Life Science, Dongguk University-Seoul, Goyang 10326, Republic of Korea.
Int J Syst Evol Microbiol. 2024 Jun;74(6). doi: 10.1099/ijsem.0.006426.
Two novel strains, designated APW6 and APW11, were isolated from artificial pond water, and one novel strain, designated PFR6, was isolated from a root. These strains were found to be Gram-negative, rod-shaped, motile by means of flagella, and oxidase-positive. Growth conditions of the type strains were as follows: APW6, 15-43 °C (optimum, 28 °C), pH 6.0-12.0 (optimum, pH 7.0), with no salinity; APW11, 4-35 °C (optimum, 25 °C), pH 6.0-11.0 (optimum, pH 9.0), with 0-1 % NaCl (w/v, optimum 0 %); PFR6, 10-38 °C (optimum 28 °C), pH 6.0-12.0 (optimum, pH 7.0), with 0-2 % NaCl (w/v; optimum, 0 %). Strains APW6, APW11, and PFR6 belonged to the genus , having the most 16S rRNA gene sequence similarity to DSM 654 (98.1 %), CHU3 (98.7 %), and CCUG 52769 (98.1 %). The estimated genome sizes of APW6, APW11, and PFR6 were 50 50 473, 56 70 008, and 52 16 869 bp, respectively and the G+C contents were 69.5, 66, and 68.5 mol%. The digital DNA-DNA hybridization, average amino acid identity, and average nucleotide identity values among the novel strains and related taxa were all lower than 22.4, 74.7, and 78.9 %, respectively. The predominant cellular fatty acids (>10 %) of all strains were summed feature 3 (comprising C 6 and/or C 7) and C. PFR6 also had summed feature 8 (comprising C 7 and/or C 6) as a major fatty acid. The polar lipid profile of all strains contained phosphatidylethanolamine, phosphoaminoglycolipid, and phosphoglycolipid. The distinct phylogenetic, physiological, and chemotaxonomic features reported in this study indicate that strains APW6, APW11, and PFR6 represent novel species within the genus , for which the names sp. nov., with the type strain APW6 (=KACC 22877=TBRC 16606), sp. nov., with the type strain APW11 (=KACC 22878=TBRC 16607), and sp. nov (=KACC 23257=TBRC 17653) are respectively proposed.
两株新菌株，分别命名为 APW6 和 APW11，从人工池塘水中分离得到，一株新菌株，命名为 PFR6，从根部分离得到。这些菌株被发现为革兰氏阴性、杆状、通过鞭毛运动、氧化酶阳性。模式菌株的生长条件如下：APW6，15-43°C（最佳，28°C），pH 6.0-12.0（最佳，pH 7.0），无盐度；APW11，4-35°C（最佳，25°C），pH 6.0-11.0（最佳，pH 9.0），0-1%NaCl（w/v，最佳 0%）；PFR6，10-38°C（最佳，28°C），pH 6.0-12.0（最佳，pH 7.0），0-2%NaCl（w/v；最佳，0%）。菌株 APW6、APW11 和 PFR6 属于属，与 DSM 654（98.1%）、CHU3（98.7%）和 CCUG 52769（98.1%）的 16S rRNA 基因序列最相似。APW6、APW11 和 PFR6 的估计基因组大小分别为 5050473、5670008 和 5216869bp，GC 含量分别为 69.5%、66%和 68.5%mol%。新型菌株与相关分类群之间的数字 DNA-DNA 杂交、平均氨基酸同一性和平均核苷酸同一性值均低于 22.4%、74.7%和 78.9%。所有菌株的主要细胞脂肪酸（>10%）均为特征 3（包含 C6 和/或 C7）和 C。PFR6 还具有特征 8（包含 C7 和/或 C6）作为主要脂肪酸。所有菌株的极性脂谱均含有磷脂酰乙醇胺、磷酸氨基糖脂和磷酸糖脂。本研究报道的独特的系统发育、生理和化学分类特征表明，菌株 APW6、APW11 和 PFR6 代表属内的新型物种，分别命名为 sp. nov.，其模式菌株为 APW6（=KACC 22877=TBRC 16606）， sp. nov.，其模式菌株为 APW11（=KACC 22878=TBRC 16607）和 sp. nov.（=KACC 23257=TBRC 17653）。
