Guzman-Tordecilla Maria, Pacheco-Bustos Carlos, Coronado-Posada Nadia, Pedrosa-Gomes Marcelo, Martinez-Burgos Walter J, Mejía-Marchena Ricardo, Zorman-Marques Raizza
Instituto de Estudios Hidráulicos y Ambientales (IDEHA), Departamento de Ingeniería Civil y Ambiental, Universidad del Norte, km 5 antigua vía a Puerto Colombia, Barranquilla, C.P. 081007, Colombia.
Instituto de Estudios Hidráulicos y Ambientales (IDEHA), Departamento de Ingeniería Civil y Ambiental, Universidad del Norte, km 5 antigua vía a Puerto Colombia, Barranquilla, C.P. 081007, Colombia.
Environ Res. 2024 Oct 1;258:119409. doi: 10.1016/j.envres.2024.119409. Epub 2024 Jun 11.
Meropenem is a potent carbapenem antibiotic frequently used in medical settings. Several studies have confirmed the pervasive presence of these antibiotics in wastewater treatment plants and aquatic environments. However, the effects of these substances on non-target organisms, such as plants, have not been adequately monitored. Thus, this study aimed to assess the short-term impact of meropenem on the growth, photosynthesis, chlorophyll content, and enzyme activity of the macrophyte plant Lemna minor. The methods involved exposing the plant to meropenem under controlled conditions and assessing physiological and biochemical parameters to determine the impact on photosynthetic activity and oxidative stress. These analyses included growth rate, antioxidant enzyme activity, and photosynthetic capacity. The findings suggest that the growth rate of Lemna minor remained unaffected by meropenem at concentrations <200000 μgL. However, plants exposed to concentrations >20 μgLshowed physiological alterations, such as decreased net photosynthesis rate (17%) and chlorophyll concentration (57%), compared to the control group. For acute toxicity assays, the calculated EC 7-day and EC 7-day were 1135 μgLand 33 μgL, respectively. In addition, in most treatments tested, meropenem caused an increase in the superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) activity as a defense mechanism against oxidative stress. Our results suggest that meropenem affects photosynthetic processes and induces oxidative stress in the macrophyte plant Lemna minor. Further studies are needed to assess the physiological and metabolic interactions between antibiotics and primary producers at different long-term trophic levels.
美罗培南是一种强效碳青霉烯类抗生素，常用于医疗环境。多项研究证实了这些抗生素在污水处理厂和水生环境中普遍存在。然而，这些物质对非靶标生物（如植物）的影响尚未得到充分监测。因此，本研究旨在评估美罗培南对大型植物浮萍生长、光合作用、叶绿素含量和酶活性的短期影响。方法包括在受控条件下将植物暴露于美罗培南，并评估生理和生化参数，以确定对光合活性和氧化应激的影响。这些分析包括生长速率、抗氧化酶活性和光合能力。研究结果表明，在浓度<200000μg/L时，美罗培南对浮萍的生长速率没有影响。然而，与对照组相比，暴露于浓度>20μg/L的植物出现了生理变化，如净光合速率降低（17%）和叶绿素浓度降低（57%）。对于急性毒性试验，计算得出的7天半数有效浓度（EC₅₀）和7天最低观察效应浓度（LOEC）分别为1135μg/L和33μg/L。此外，在大多数测试处理中，美罗培南导致超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX）活性增加，作为对抗氧化应激的防御机制。我们的结果表明，美罗培南影响大型植物浮萍的光合过程并诱导氧化应激。需要进一步研究来评估抗生素与不同长期营养水平的初级生产者之间的生理和代谢相互作用。
