Cornelissen A W, Langsley G, Walliker D, Scaife J G
Mol Biochem Parasitol. 1985 Feb;14(2):165-74. doi: 10.1016/0166-6851(85)90035-0.
A cloned Plasmodium berghei (ANKA) isolate was syringe passaged repeatedly to generate a line that was non-infective to Anopheles stephensi. Ribosomal gene organisation of this non-infective line was then compared to its infective ancestor. DNA was also prepared from asexual parasites and gametocytes of P. chabaudi and the arrangement of the rRNA genes of this species was studied. Although macrogametocytes have many more ribosomes than microgametocytes, this increase does not appear to stem from an amplification of the rRNA genes, as no differences either in the quantity or the arrangement of the rDNA could be detected. Furthermore, the loss of infectivity of the P. berghei gametocytes does not seem to be due to a reduction or rearrangement of sequences coding for the rRNA genes. P. chabaudi and P. berghei DNA failed to show any homology to a repetitive DNA sequence cloned from P. falciparum. We conclude that this probe, PFH8rep20, is specific for P. falciparum.
将克隆的伯氏疟原虫（ANKA株）分离株通过注射器反复传代，以获得对斯氏按蚊无感染性的品系。然后将该无感染性品系的核糖体基因组织与其有感染性的祖先进行比较。还从查巴迪疟原虫的无性寄生虫和配子体中制备了DNA，并研究了该物种rRNA基因的排列。尽管大配子体的核糖体比小配子体多得多，但这种增加似乎并非源于rRNA基因的扩增，因为在rDNA的数量或排列上均未检测到差异。此外，伯氏疟原虫配子体感染性的丧失似乎并非由于编码rRNA基因的序列减少或重排所致。查巴迪疟原虫和伯氏疟原虫的DNA未显示与从恶性疟原虫克隆的重复DNA序列有任何同源性。我们得出结论，该探针PFH8rep20对恶性疟原虫具有特异性。
