Potter T A, Zeff R A, Schmitt-Verhulst A M, Rajan T V
Proc Natl Acad Sci U S A. 1985 May;82(9):2950-4. doi: 10.1073/pnas.82.9.2950.
EL4/Mar is a variant cell line that expresses H-2Db but neither H-2Kb nor beta 2-microglobulin (beta 2m). Southern and RNA blot analysis and immunoprecipitation of metabolically labeled proteins established that the B2m gene(s), beta 2m mRNA, and beta 2m protein are normal in this cell line. Somatic cell hybridization showed that the defect in this cell line was in the synthesis of H-2Kb, and RNA blot analysis with an H-2Kb specific oligonucleotide established that the H-2Kb gene(s) in this cell line was not transcribed into a stable mRNA species. The apparent absence of beta 2m on the surface of this cell line suggests that there may be some feature of the H-2Db molecule that allows it to be expressed in the absence of detectable beta 2m.
EL4/Mar是一种变异细胞系，它表达H-2Db，但既不表达H-2Kb也不表达β2-微球蛋白（β2m）。Southern印迹分析、RNA印迹分析以及对代谢标记蛋白的免疫沉淀表明，该细胞系中的B2m基因、β2m mRNA和β2m蛋白均正常。体细胞杂交显示，该细胞系的缺陷在于H-2Kb的合成，并且用H-2Kb特异性寡核苷酸进行的RNA印迹分析表明，该细胞系中的H-2Kb基因未转录成稳定的mRNA种类。该细胞系表面明显缺乏β2m表明，H-2Db分子可能具有某些特性，使其能够在未检测到β2m的情况下表达。
