State Key Laboratory of Plant Environmental Resilience, Frontiers Science Center for Molecular Design Breeding, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
School of Life Science, Institute of Life Science and Green Development, Hebei University, Baoding, Hebei 071002, China.
Plant Cell. 2024 Oct 3;36(10):4356-4371. doi: 10.1093/plcell/koae177.
Plants respond to cold stress at multiple levels, including increasing cytosolic calcium (Ca2+) influx and triggering the expression of cold-responsive genes. In this study, we show that the Ca2+-permeable channel CYCLIC NUCLEOTIDE-GATED CHANNEL20 (CNGC20) positively regulates freezing tolerance in Arabidopsis (Arabidopsis thaliana) by mediating cold-induced Ca2+ influx. Moreover, we demonstrate that the leucine-rich repeat receptor-like kinase PLANT PEPTIDE CONTAINING SULFATED TYROSINE1 RECEPTOR (PSY1R) is activated by cold, phosphorylating and enhancing the activity of CNGC20. The psy1r mutant exhibits decreased cold-evoked Ca2+ influx and freezing tolerance. Conversely, COLD-RESPONSIVE PROTEIN KINASE1 (CRPK1), a protein kinase that negatively regulates cold signaling, phosphorylates and facilitates the degradation of CNGC20 under prolonged periods of cold treatment, thereby attenuating freezing tolerance. This study thus identifies PSY1R and CRPK1 kinases that regulate CNGC20 activity and stability, respectively, thereby antagonistically modulating freezing tolerance in plants.
植物在多个层面上对冷胁迫做出反应，包括增加细胞质钙离子（Ca2+）内流并触发冷响应基因的表达。在这项研究中，我们表明钙通透通道环核苷酸门控通道 20（CNGC20）通过介导冷诱导的 Ca2+内流，正向调节拟南芥（Arabidopsis thaliana）的抗冻性。此外，我们证明富含亮氨酸重复的受体样激酶植物肽含硫酸酪氨酸 1 受体（PSY1R）被冷激活，磷酸化并增强 CNGC20 的活性。psy1r 突变体表现出冷诱导 Ca2+内流减少和抗冻性降低。相反，冷响应蛋白激酶 1（CRPK1）是一种负调控冷信号的蛋白激酶，在长时间的冷处理下，磷酸化并促进 CNGC20 的降解，从而减弱抗冻性。因此，本研究鉴定了 PSY1R 和 CRPK1 激酶，它们分别调节 CNGC20 的活性和稳定性，从而拮抗调节植物的抗冻性。
