National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; Hubei Hongshan Laboratory, Wuhan 430070, China.
Department of Molecular Physiology, Max- Planck- Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany; Center of Plant Systems Biology and Biotechnology, 4000 Plovdiv, Bulgaria.
Mol Plant. 2024 Jul 1;17(7):1005-1018. doi: 10.1016/j.molp.2024.06.005. Epub 2024 Jun 13.
Doubled haploid (DH) technology and synthetic apomixis approaches can considerably shorten breeding cycles and enhance breeding efficiency. Compared with traditional breeding methods, DH technology offers the advantage of rapidly generating inbred lines, while synthetic apomixis can effectively fix hybrid vigor. In this review, we focus on (i) recent advances in identifying and characterizing genes responsible for haploid induction (HI), (ii) the molecular mechanisms of HI, (iii) spontaneous haploid genome doubling, and (iv) crop synthetic apomixis. We also discuss the challenges and potential solutions for future crop breeding programs utilizing DH technology and synthetic apomixis. Finally, we provide our perspectives about how to integrate DH and synthetic apomixis for precision breeding and de novo domestication.
加倍单倍体 (DH) 技术和人工合成无融合生殖方法可以大大缩短育种周期,提高育种效率。与传统的育种方法相比,DH 技术具有快速产生纯系的优势,而人工合成无融合生殖可以有效地固定杂种优势。在这篇综述中,我们重点介绍了 (i) 鉴定和描述负责单倍体诱导 (HI) 的基因的最新进展,(ii) HI 的分子机制,(iii) 自发单倍体基因组加倍,和 (iv) 作物人工合成无融合生殖。我们还讨论了未来利用 DH 技术和人工合成无融合生殖进行作物育种计划的挑战和潜在解决方案。最后,我们就如何整合 DH 和人工合成无融合生殖进行精准育种和从头驯化提出了自己的看法。