Key Laboratory of Carbohydrate Science and Engineering, Chongqing Normal University, Chongqing 401331, China.
Key Laboratory of Carbohydrate Science and Engineering, Chongqing Normal University, Chongqing 401331, China.
Ultrason Sonochem. 2024 Aug;108:106948. doi: 10.1016/j.ultsonch.2024.106948. Epub 2024 Jun 11.
In this study, the process conditions, physicochemical properties, structural composition and activity of polysaccharides isolated from leechee peel (LPP) by ultrasound-assisted extraction (UAE) with enzyme and alkali solution extraction (ASE) were compared. The results showed that the total sugar content of LPP extracted by UAE accounted for 75.65 %, which was significantly higher than that extracted by alkali solution. The optimum conditions were as follows: extraction temperature of 68.78 ℃, ultrasonic enzymolysis time of 39.68 min, pectinase dosage of 4.03 %, solid-liquid ratio of 1:30 g/mL, and ultrasonic power of 360 W. The antioxidant activities and structure of leechee peel polysaccharide (LPP) prepared under different conditions were compared. It was found that UAE-LPP was an α-type polysaccharide containing 15.83 % uronic acid. Moreover, LPP extracted by UAE showed strong activity in anti-lipid peroxidation and reducing ability. Ultrasound-assisted enzymatic method is an effective means to improve the content and activity of natural plant polysaccharides, and this method has the advantages of short time-consuming, simple process and easy operation, which can greatly improve the utilization rate of polysaccharides and lay a theoretical and scientific basis for the development and utilization of LPP.
在这项研究中，通过超声辅助提取（UAE）与酶和碱溶液提取（ASE）对比，研究了从荔枝皮（LPP）中分离出的多糖的工艺条件、物理化学性质、结构组成和活性。结果表明，UAE 提取的荔枝皮总糖含量占 75.65%，明显高于碱溶液提取的总糖含量。最佳条件为：提取温度 68.78℃，超声酶解时间 39.68min，果胶酶用量 4.03%，固液比 1:30g/mL，超声功率 360W。比较了不同条件下制备的荔枝皮多糖（LPP）的抗氧化活性和结构。结果表明，UAE-LPP 是一种含有 15.83%的糖醛酸的α型多糖。此外，UAE 提取的 LPP 具有较强的抗脂质过氧化和还原能力。超声辅助酶法是提高天然植物多糖含量和活性的有效手段，该方法具有耗时短、工艺简单、操作方便等优点，可大大提高多糖的利用率，为 LPP 的开发利用奠定理论和科学基础。
