Yang Minghui, Li Xingfan, Du Xinyi, Li Falin, Wang Tianqi, Gao Yanyan, Liu Jia, Luo Xiongfei, Guo Xiaorui, Tang Zhonghua
College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, China; Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China.
Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150040, China.
Ultrason Sonochem. 2025 Jul;118:107364. doi: 10.1016/j.ultsonch.2025.107364. Epub 2025 Apr 21.
Modern pharmacological investigations have shown that polysaccharides extracted from Boschniakia rossica showcase a range of biological effects. This study identified 260 primary metabolites, with carbohydrate compounds and their derivatives accounting for 80 types. We employed ultrasonic-assisted enzyme extraction (UAEE) to optimize extraction parameters, achieving a crude polysaccharide yield of 13.67%, which is 1.52 times and 1.17 times higher than ultrasonic extraction (UAE) and enzyme extraction (EAE). Additionally, we isolated four distinct purified polysaccharides (BRPS-0, BRPS-1, BRPS-2, BRPS-3), with relative molecular weights of 1569, 2815, and 4572, while BRPS-3 displayed structural complexity. The scanning electron microscope (SEM) revealed that BRPS-0 exhibited distinct crystalline particles, while BRPS-3 displayed an ordered crystal structure. Notably, BRPS-0 demonstrated relatively stable antioxidant activity due to its low molecular weight, high phenolic and sugar content, crystalline microstructure, and abundant α- and β-pyranose configurations, including galacturonic acid, glucose, galactose, and arabinose. This research provides a foundational theory for the comprehensive use of Boschniakia rossica resources and supports its implementation in modern medicine and functional foods.
现代药理学研究表明，从草苁蓉中提取的多糖具有一系列生物学效应。本研究鉴定出260种初级代谢产物，其中碳水化合物及其衍生物有80种。我们采用超声辅助酶提取法（UAEE）优化提取参数，粗多糖得率达到13.67%，分别比超声提取法（UAE）和酶提取法（EAE）高1.52倍和1.17倍。此外，我们分离出四种不同的纯化多糖（BRPS - 0、BRPS - 1、BRPS - 2、BRPS - 3），相对分子量分别为1569、2815和4572，而BRPS - 3的结构较为复杂。扫描电子显微镜（SEM）显示，BRPS - 0呈现出明显的结晶颗粒，而BRPS - 3具有有序的晶体结构。值得注意的是，BRPS - 0因其分子量低、酚类和糖类含量高、结晶微观结构以及丰富的α - 和β - 吡喃糖构型（包括半乳糖醛酸、葡萄糖、半乳糖和阿拉伯糖）而表现出相对稳定的抗氧化活性。本研究为草苁蓉资源的综合利用提供了基础理论，并支持其在现代医学和功能性食品中的应用。
