Optometry Division, Department of Allied Health Sciences, Faculty of Life and Allied Health Sciences, M S Ramaiah University of Applied Sciences, Bengaluru, India.
Department of Pharmaceuticals, Faculty of Pharmacy, M S Ramaiah University of Applied Sciences, Bengaluru, India.
Eur J Med Res. 2024 Jun 16;29(1):332. doi: 10.1186/s40001-024-01927-z.
Glaucoma is the most common cause of irreversible blindness in the world. It is associated with elevated intraocular pressure (IOP). Fluctuations in tonometer readings have implications for glaucoma research, where accurate IOP measurements are vital for evaluating disease progression and treatment efficacy. Researchers should carefully select the appropriate tonometer and consider biases associated with different tonometers. Validation against standard measurements can improve IOP measurement accuracy in rat models. In conclusion, this systematic review will emphasize on the importance of selecting the appropriate tonometer for IOP measurement in rat models, considering potential biases and their implications for glaucoma research. Accurate and consistent IOP measurement in rat models is crucial for understanding glaucoma pathophysiology and developing effective treatments. This systematic review aims to assess agreement among tonometers used for measuring IOP in Wistar rat models primarily focusing on TonoLab, TonoVet, and Tono-pen. The review was conducted using PRISMA guidelines. Two articles were included for qualitative synthesis. The studies compared manometric IOP with TonoLab, rebound tonometer, and Tono-pen XL readings. It was observed that TonoLab consistently underestimated IOP, while Tono-pen XL tended to overestimate IOP compared to manometric measurements. The study's findings will help researchers in making decisions about tonometer selection, leading to more reliable outcomes in glaucoma research using rat models. Further research, specifically RCT's (randomized controlled trial) is needed to confirm the results and enhance IOP measurement precision in rat models.
青光眼是世界上最常见的不可逆性失明原因。它与眼内压（IOP）升高有关。眼压计读数的波动对青光眼研究有影响，在青光眼研究中，准确的 IOP 测量对于评估疾病进展和治疗效果至关重要。研究人员应仔细选择合适的眼压计，并考虑不同眼压计的偏差。与标准测量值进行验证可以提高大鼠模型中 IOP 测量的准确性。总之，本系统评价将强调在大鼠模型中选择合适的眼压计进行 IOP 测量的重要性，考虑潜在的偏差及其对青光眼研究的影响。在大鼠模型中准确和一致地测量 IOP 对于理解青光眼病理生理学和开发有效的治疗方法至关重要。本系统评价旨在评估主要用于测量 Wistar 大鼠模型中 IOP 的眼压计（TonoLab、TonoVet 和 Tono-pen）之间的一致性。该综述使用 PRISMA 指南进行。纳入了两篇用于定性综合的文章。这些研究将眼压计与眼压计、回弹眼压计和 Tono-pen XL 的测量值进行了比较。结果表明，TonoLab 始终低估了 IOP，而 Tono-pen XL 与眼压计测量值相比，往往高估了 IOP。该研究的结果将有助于研究人员在眼压计选择方面做出决策，从而在使用大鼠模型进行青光眼研究中获得更可靠的结果。需要进一步的研究，特别是 RCT（随机对照试验），以确认结果并提高大鼠模型中 IOP 测量的精度。
