Kuznetsov V D, Bushueva O A, Gorin S E
Antibiot Med Biotekhnol. 1985 Jan;30(1):27-30.
The results of the study on long-term maintenance (1-12 years) of 453 reference cultures of actinomycetes are presented. The cultures were maintained with the following methods: lyophilization, soil cultures and periodical passages on agarized nutrient media. Lyophilization was most efficient for storage of the majority of the cultures: 346 strains (77.4 per cent) preserved high viability over the observation period of 11-12 years. Lyophilization appeared less favourable for the cultures with low sporulation. The efficacy of the method of soil cultures on long-term storage of the whorl actinomycetes was low: 88 per cent of Streptoverticillium lost their viability within 1-2 years. However, this method was valuable for stabilization of the taxonomic features of streptomycetes. The maintenance of the actinomycetes by regular passages on agarized media every 6 months resulted in the changing of the cultural and morphological features in a number of the strains.
本文展示了对453株放线菌参考菌株进行1至12年长期保存的研究结果。这些菌株采用以下方法保存：冻干法、土壤培养法以及在琼脂营养培养基上定期传代培养。对于大多数菌株的保存而言，冻干法最为有效：在11至12年的观察期内，346株菌株（77.4%）保持了高活力。冻干法对于产孢率低的菌株似乎不太适用。轮生放线菌的土壤培养法在长期保存方面效果不佳：88%的轮枝链霉菌在1至2年内失去活力。然而，该方法对于稳定链霉菌的分类特征具有重要价值。每6个月在琼脂培养基上定期传代培养放线菌，导致许多菌株的培养和形态特征发生变化。
