Malaiappan Sankari, P T Priyangha, Niveditha Sankari
Department of Periodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IND.
Department of Dentistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IND.
Cureus. 2024 May 16;16(5):e60407. doi: 10.7759/cureus.60407. eCollection 2024 May.
Nanotechnology enables precise manipulation of matter at the molecular level, with nanoparticles offering diverse applications in medicine and beyond. Green synthesis methods, utilizing natural sources like plant extracts, are favored for their eco-friendliness. Zinc oxide (ZnO) nanoparticles are recognized for their ability to combat microbes and reduce inflammation, which holds promise for biomedical applications. , renowned for its medicinal properties, warrants further exploration in oral health management due to its anti-inflammatory and antioxidant attributes.
The current study aimed to synthesize -mediated ZnO nanoparticles and to evaluate their anti-inflammatory and antioxidant activity.
powder (1 g) was dissolved in distilled water (100 ml), heated at 60°C for 15-20 minutes, and filtered to obtain 20 ml extract. ZnO nanoparticles were synthesized by adding 0.594 g ZnO powder to 50 ml water, mixed with plant extract, and stirred for 72 hours, and the resulting solution was centrifuged. Nanoparticles were collected and analyzed for Fourier-transform infrared spectroscopy (FTIR) using Bruker's Alpha II FTIR spectrometer (Bruker, Billerica, Massachusetts, United States), antioxidant, and anti-inflammatory activities.
FTIR analysis revealed characteristic peaks indicative of functional groups present in -mediated ZnO nanoparticles, including O-H, N-O, C-O, C=C, and C≡C-H. Anti-inflammatory activity evaluation showed inhibition ranging from 48% to 89%, with the maximum inhibition at 50 μL concentration. Similarly, antioxidant activity ranged from 62% to 88%, with the maximum inhibition also seen at 50 μL concentration.
Both assays effectively showcased the superior anti-inflammatory and antioxidant activity of the -incorporated ZnO nanoparticles extract compared to the control. This suggests their potential as a viable therapeutic agent for further evaluation.
纳米技术能够在分子水平上精确操纵物质，纳米颗粒在医学及其他领域有着广泛应用。利用植物提取物等天然来源的绿色合成方法因其环保性而受到青睐。氧化锌（ZnO）纳米颗粒因其对抗微生物和减轻炎症的能力而受到认可，这为生物医学应用带来了希望。因其药用特性，由于其抗炎和抗氧化特性，在口腔健康管理方面值得进一步探索。
本研究旨在合成介导的ZnO纳米颗粒，并评估其抗炎和抗氧化活性。
将粉末（1 g）溶解于蒸馏水（100 ml）中，在60°C加热15 - 20分钟，过滤得到20 ml提取物。通过将0.594 g ZnO粉末加入50 ml水中，与植物提取物混合并搅拌72小时来合成ZnO纳米颗粒，然后将所得溶液离心。收集纳米颗粒，使用布鲁克的Alpha II傅里叶变换红外光谱仪（FTIR）（布鲁克，美国马萨诸塞州比勒里卡）分析其傅里叶变换红外光谱（FTIR）、抗氧化和抗炎活性。
FTIR分析揭示了介导的ZnO纳米颗粒中存在的官能团的特征峰，包括O - H、N - O、C - O、C = C和C≡C - H。抗炎活性评估显示抑制率在48%至89%之间，在50 μL浓度时抑制作用最大。同样，抗氧化活性在62%至88%之间，在50 μL浓度时也观察到最大抑制作用。
两种测定方法均有效展示了与对照相比，掺入的ZnO纳米颗粒提取物具有优异的抗炎和抗氧化活性。这表明它们作为一种可行的治疗剂有待进一步评估的潜力。
