Scheuermann D W, Stach W
Histochemistry. 1985;82(3):269-73. doi: 10.1007/BF00501404.
The myenteric plexus of the porcine small intestine is studied using a combined method for the simultaneous visualization of enteric intramural neuronal cell bodies and peptidergic nerve fibers. As earlier reported, the histochemical method for demonstration of the NADH-dependent dehydrogenase reaction allows the identification of the three neuron types of Dogiel but, in addition, the afore mentioned staining method creates fair conditions in the tissue for the subsequent indirect immunocytochemical visualization of neuropeptides, as demonstrated in this work by means of the indirect immunofluorescence method for enkephalin-like immunoreactivity. Intense fluorescent varicosities of enkephalin-like nerve fibres were found to ramify around dark-blue stained ganglionic cells of type I, type II and type III in a manner suggestive of innervation.
采用一种联合方法对猪小肠的肌间神经丛进行研究，该方法可同时观察肠壁内神经元细胞体和肽能神经纤维。如先前报道，用于显示依赖烟酰胺腺嘌呤二核苷酸（NADH）的脱氢酶反应的组织化学方法能够识别多吉尔（Dogiel）三种神经元类型，此外，如本研究通过脑啡肽样免疫反应性的间接免疫荧光法所示，上述染色方法为后续神经肽的间接免疫细胞化学观察在组织中创造了良好条件。发现脑啡肽样神经纤维的强烈荧光膨体以一种提示神经支配的方式在深蓝色染色的I型、II型和III型神经节细胞周围分支。
