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马铃薯和啤酒花花粉用于冷冻保存及杂交育种的热特性分析

Analysis of Thermal Characteristics of Potato and Hop Pollen for Their Cryopreservation and Cross-Breeding.

作者信息

Faltus Milos, Domkářová Jaroslava, Svoboda Petr, Horáčková Vendulka, Nesvadba Vladimír, Klička Vladislav, Ptáček Jiří, Bilavcik Alois, Zamecnik Jiri

机构信息

Crop Research Institute, Drnovská 507, CZ161 06 Prague, Czech Republic.

Potato Research Institute, Dobrovského 2366, CZ580 01 Havlickuv Brod, Czech Republic.

出版信息

Plants (Basel). 2024 Jun 6;13(11):1578. doi: 10.3390/plants13111578.

DOI:10.3390/plants13111578
PMID:38891386
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11174463/
Abstract

This study investigated the thermal properties of potato and hop pollen for cryopreservation and subsequent cross-breeding. Phase transitions and frozen water content in selected pollen samples were measured using a differential scanning calorimeter (DSC). Unlike hop pollen, potato pollen showed high variability in thermal properties and water content. Three specific types of pollen samples based on their thermal characteristics and water content were distinguished by DSC in potato: (1) 'glassy', with a water content lower than 0.21 g water per g dry matter; (2) 'transient', with a water content between 0.27 and 0.34 g of water per g of dry matter; (3) 'frozen', with a water content higher than 0.34 g of water per g of dry matter. Only the 'glassy' pollen samples with a low water content showed suitable properties for its long-term storage using cryopreservation in potato and hops. Cryopreservation of pollen did not significantly reduce its viability, and cryopreserved pollen was successfully used to produce both potato and hop hybrids. The results indicate that cryopreservation is a feasible technique for the preservation and utilization of pollen of these crops in the breeding process.

摘要

本研究调查了马铃薯和啤酒花花粉的热特性，以用于冷冻保存及后续杂交育种。使用差示扫描量热仪（DSC）测量选定花粉样本中的相变和冷冻水含量。与啤酒花花粉不同，马铃薯花粉的热特性和含水量表现出高度变异性。通过DSC在马铃薯中区分出了基于热特性和含水量的三种特定类型的花粉样本：（1）“玻璃态”，含水量低于每克干物质0.21克水；（2）“过渡态”，含水量在每克干物质0.27至0.34克水之间；（3）“冷冻态”，含水量高于每克干物质0.34克水。只有含水量低的“玻璃态”花粉样本表现出适合在马铃薯和啤酒花中使用冷冻保存进行长期储存的特性。花粉的冷冻保存并未显著降低其活力，并且冷冻保存的花粉成功用于培育马铃薯和啤酒花杂交品种。结果表明，冷冻保存是在育种过程中保存和利用这些作物花粉的可行技术。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9747/11174463/7f59642d068b/plants-13-01578-g014.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9747/11174463/6a1c908e96a4/plants-13-01578-g002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9747/11174463/d10fd3d7a4f8/plants-13-01578-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9747/11174463/5713e0eef0ad/plants-13-01578-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9747/11174463/1902ecc0b5a1/plants-13-01578-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9747/11174463/f205eb998b73/plants-13-01578-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9747/11174463/7df91d66a901/plants-13-01578-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9747/11174463/b9663c52104b/plants-13-01578-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9747/11174463/776bd15ff8aa/plants-13-01578-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9747/11174463/a175d1e64ed0/plants-13-01578-g011.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9747/11174463/ec1a8c5444d6/plants-13-01578-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9747/11174463/7f59642d068b/plants-13-01578-g014.jpg

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Genetic Diversity, Conservation, and Utilization of Plant Genetic Resources.植物遗传资源的遗传多样性、保护和利用。
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Cytoplasmic Male Sterility Incidence in Potato Breeding Populations with Late Blight Resistance and Identification of Breeding Lines with a Potential Fertility Restorer Mechanism.具有晚疫病抗性的马铃薯育种群体中的细胞质雄性不育发生率及具有潜在育性恢复机制的育种系鉴定
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