Department of Industrial Engineering, University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano, Italy.
Molecules. 2024 May 25;29(11):2498. doi: 10.3390/molecules29112498.
Bone tissue engineering (BTE) is the most promising strategy to repair bones injuries and defects. It relies on the utilization of a temporary support to host the cells and promote nutrient exchange (i.e., the scaffold). Supercritical CO assisted drying can preserve scaffold nanostructure, crucial for cell attachment and proliferation. In this work, agarose aerogels, loaded with hydroxyapatite were produced in view of BTE applications. Different combinations of agarose concentration and hydroxyapatite loadings were tested. FESEM and EDX analyses showed that scaffold structure suffered from partial closure when increasing filler concentration; hydroxyapatite distribution was homogenous, and Young's modulus improved. Looking at BTE applications, the optimal combination of agarose and hydroxyapatite resulted to be 1% / and 10% /, respectively. Mechanical properties showed that the produced composites could be eligible as starting scaffold for BTE, with a Young's Modulus larger than 100 kPa for every blend.
骨组织工程（BTE）是修复骨骼损伤和缺陷最有前途的策略。它依赖于利用临时支架来容纳细胞并促进营养物质交换（即支架）。超临界 CO 辅助干燥可以保留支架的纳米结构，这对细胞附着和增殖至关重要。在这项工作中，考虑到 BTE 的应用，制备了负载羟基磷灰石的琼脂糖气凝胶。测试了琼脂糖浓度和羟基磷灰石负载量的不同组合。FESEM 和 EDX 分析表明，当填充剂浓度增加时，支架结构会部分封闭；羟基磷灰石分布均匀，杨氏模量提高。着眼于 BTE 的应用，琼脂糖和羟基磷灰石的最佳组合分别为 1%/和 10%/。机械性能表明，所制备的复合材料可作为 BTE 的起始支架，每种共混物的杨氏模量均大于 100 kPa。
