Paczkowska-Walendowska Magdalena, Ignacyk Miłosz, Miklaszewski Andrzej, Plech Tomasz, Karpiński Tomasz M, Kwiatek Jakub, Swora-Cwynar Ewelina, Walendowski Michał, Cielecka-Piontek Judyta
Department of Pharmacognosy and Biomaterials, Poznan University of Medical Sciences, Rokietnicka 3, 60-806 Poznan, Poland.
Faculty of Materials Engineering and Technical Physics, Institute of Materials Science and Engineering, Poznan University of Technology, 60-965 Poznan, Poland.
Materials (Basel). 2024 May 26;17(11):2558. doi: 10.3390/ma17112558.
Pomegranate peel extract is known for its potent antibacterial, antiviral, antioxidant, anti-inflammatory, wound healing, and probiotic properties, leading to its use in treating oral infections. In the first stage of this work, for the first time, using the Design of Experiment (DoE) approach, pomegranate peel extract (70% methanol, temperature 70 °C, and three cycles per 90 min) was optimized and obtained, which showed optimal antioxidant and anti-inflammatory properties. The optimized extract showed antibacterial activity against oral pathogenic bacteria. The second part of this study focused on optimizing an electrospinning process for a combination of polycaprolactone (PCL) and polyvinylpyrrolidone (PVP) nanofibers loaded with the optimized pomegranate peel extract. The characterization of the nanofibers was confirmed by using SEM pictures, XRPD diffractograms, and IR-ATR spectra. The composition of the nanofibers can control the release; in the case of PVP-based nanofibers, immediate release was achieved within 30 min, while in the case of PCL/PVP, controlled release was completed within 24 h. Analysis of the effect of different scaffold compositions of the obtained electrofibers showed that those based on PCL/PVP had better wound healing potential. The proposed strategy to produce electrospun nanofibers with pomegranate peel extract is the first and innovative approach to better use the synergy of biological action of active compounds present in extracts in a patient-friendly pharmaceutical form, beneficial for treating oral infections.
石榴皮提取物以其强大的抗菌、抗病毒、抗氧化、抗炎、伤口愈合和益生菌特性而闻名，因此被用于治疗口腔感染。在这项工作的第一阶段，首次采用实验设计（DoE）方法，对石榴皮提取物（70%甲醇、70°C温度、每90分钟三个循环）进行了优化并获得了结果，该提取物表现出最佳的抗氧化和抗炎特性。优化后的提取物对口腔病原菌具有抗菌活性。本研究的第二部分重点是优化一种静电纺丝工艺，用于制备负载优化后的石榴皮提取物的聚己内酯（PCL）和聚乙烯吡咯烷酮（PVP）纳米纤维组合。通过扫描电子显微镜（SEM）图片、X射线粉末衍射（XRPD）图谱和红外衰减全反射（IR-ATR）光谱对纳米纤维进行了表征。纳米纤维的组成可以控制释放；对于基于PVP的纳米纤维，在30分钟内实现了即时释放，而对于PCL/PVP纳米纤维，控释在24小时内完成。对所得电纺纤维不同支架组成的效果分析表明，基于PCL/PVP的电纺纤维具有更好的伤口愈合潜力。所提出的制备负载石榴皮提取物的电纺纳米纤维的策略是第一种创新性方法，能够以患者友好的药物形式更好地利用提取物中活性化合物生物作用的协同效应，有利于治疗口腔感染。
