Zhang Haiyan, Qiu Quan, Li Qingya, Feng Long, Li Dongyang, Zhang Miao, Wang Juan, Ma Suna, Xu Liran
Zhongjing Institute of Chinese Medicine, Henan University of Chinese Medicine, Zhengzhou city, Henan Province, China.
Department of Pathogenic Organism Biology, Henan University of Chinese Medicine, Zhengzhou city, Henan Province, China.
Iran J Public Health. 2024 Feb;53(2):376-386. doi: 10.18502/ijph.v53i2.14922.
We aimed to distinguish the different Chinese medicine (CM) syndromes of acquired immune deficiency syndrome (AIDS) patients at the proteomics level.
We collected AIDS patients diagnosed with different CM syndromes from Weishi County, Kaifeng City, Henan Province, China, including Qi-deficiency syndrome (named QD group) and dampness-heat syndrome (named DH group). Healthy people were collected as controls from Weishi County, Kaifeng city, Henan Province, China. The plasma from three groups were labeled with ITRAQ, LC/MC was used for protein quantitative analysis. Finally, sequence search and cluster analysis were performed.
Overall, 27 different proteins were found. Three proteins were up-regulated and 2 proteins down-regulated in the QD group, 11 proteins up-regulated and 13 proteins down-regulated in the DH group. Compared with DH group, there were 7 different proteins in QD group, among which 5 proteins were down-regulated and 2 proteins were up-regulated. When the target protein of DH group was up-regulated, the protein of HC group was down-regulated correspondingly.
The significance analysis and clustering of protein results showed that DH group was significantly different from QD group and HC group at the protein level (0.05). However, the QD group could not be effectively distinguished from the HC group. AAT, PF4, C-reactive protein and c4bp may be used as potential biomarkers in DH group. Mass spectrometry based on feature selection can be used to classify different CM syndromes.
我们旨在从蛋白质组学水平区分获得性免疫缺陷综合征（AIDS）患者的不同中医证候。
我们从中国河南省开封市尉氏县收集了诊断为不同中医证候的AIDS患者，包括气虚证（命名为QD组）和湿热证（命名为DH组）。从中国河南省开封市尉氏县收集健康人作为对照。对三组血浆进行iTRAQ标记，采用液相色谱/质谱联用技术进行蛋白质定量分析。最后进行序列搜索和聚类分析。
总体上，共发现27种不同蛋白质。QD组有3种蛋白质上调，2种蛋白质下调；DH组有11种蛋白质上调，13种蛋白质下调。与DH组相比，QD组有7种不同蛋白质，其中5种蛋白质下调，2种蛋白质上调。当DH组的靶蛋白上调时，健康对照组（HC组）的蛋白质相应下调。
蛋白质结果的显著性分析和聚类显示，DH组在蛋白质水平上与QD组和HC组有显著差异（P<0.05）。然而，QD组与HC组无法有效区分。α1抗胰蛋白酶（AAT）、血小板因子4（PF4）、C反应蛋白和补体C4结合蛋白（c4bp）可能作为DH组的潜在生物标志物。基于特征选择的质谱分析可用于不同中医证候的分类。
