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大肠杆菌K12中uidA基因调控区的核苷酸序列。

Nucleotide sequence of a regulatory region of the uidA gene in Escherichia coli K12.

作者信息

Blanco C, Ritzenthaler P, Mata-Gilsinger M

出版信息

Mol Gen Genet. 1985;199(1):101-5. doi: 10.1007/BF00327517.

DOI:10.1007/BF00327517
PMID:3889544
Abstract

Multiple regulatory events are involved in the expression of the uidA gene. A regulatory region of this gene has been located on a 460 base pair Sau3A-EcoRI fragment and its nucleotide sequence was determined by the dideoxy method using pEMBL plasmids. A preliminary analysis of this sequence revealed the presence of numerous palindromic structures with some overlaps.

摘要

uidA基因的表达涉及多个调控事件。该基因的一个调控区域位于一个460碱基对的Sau3A - EcoRI片段上，其核苷酸序列通过使用pEMBL质粒的双脱氧法测定。对该序列的初步分析揭示了存在许多有部分重叠的回文结构。

相似文献

1
Nucleotide sequence of a regulatory region of the uidA gene in Escherichia coli K12.大肠杆菌K12中uidA基因调控区的核苷酸序列。
Mol Gen Genet. 1985;199(1):101-5. doi: 10.1007/BF00327517.
2
Genetic analysis of uidA expression in enterohaemorrhagic Escherichia coli serotype O157:H7.肠出血性大肠杆菌O157:H7中uidA基因表达的遗传分析。
Microbiology (Reading). 1994 Aug;140 ( Pt 8):2101-7. doi: 10.1099/13500872-140-8-2101.
3
Cloning and endonuclease restriction analysis of uidA and uidR genes in Escherichia coli K-12: determination of transcription direction for the uidA gene.大肠杆菌K-12中uidA和uidR基因的克隆及核酸内切酶限制分析：uidA基因转录方向的确定
J Bacteriol. 1982 Feb;149(2):587-94. doi: 10.1128/jb.149.2.587-594.1982.
4
Identification of uidA gene sequences in beta-D-glucuronidase-negative Escherichia coli.在β-D-葡萄糖醛酸酶阴性大肠杆菌中鉴定uidA基因序列。
Appl Environ Microbiol. 1991 Jan;57(1):320-3. doi: 10.1128/aem.57.1.320-323.1991.
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Transcriptional and translational signals of the uidA gene in Escherichia coli K12.大肠杆菌K12中uidA基因的转录和翻译信号
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6
Negative dominant mutations of the uidR gene in Escherichia coli: genetic proof for a cooperative regulation of uidA expression.大肠杆菌中uidR基因的负显性突变：uidA基因表达协同调控的遗传学证据
Genetics. 1986 Feb;112(2):173-82. doi: 10.1093/genetics/112.2.173.
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The use of gene fusions to study the expression of uidR, a negative regulatory gene of Escherichia coli K-12.利用基因融合技术研究大肠杆菌K-12的负调控基因uidR的表达。
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Proteins. 1986 Oct;1(2):125-33. doi: 10.1002/prot.340010204.
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Plasmids with the uidA reporter gene for the detection of promoters and transcription signals.带有uidA报告基因的质粒，用于检测启动子和转录信号。
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Nucleotide sequence of a Bacillus subtilis arginine regulatory gene and homology of its product to the Escherichia coli arginine repressor.枯草芽孢杆菌精氨酸调控基因的核苷酸序列及其产物与大肠杆菌精氨酸阻遏物的同源性
Gene. 1989 Aug 1;80(1):29-38. doi: 10.1016/0378-1119(89)90247-3.

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本文引用的文献

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Cyclic AMP receptor protein: role in transcription activation.环磷酸腺苷受体蛋白：在转录激活中的作用。
Science. 1984 May 25;224(4651):831-8. doi: 10.1126/science.6372090.
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Interchangeability of repressors for the control of the uxu and uid operons in E. coli K12.大肠杆菌K12中阻遏物对uxu和uid操纵子控制的互换性。
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Compilation and analysis of Escherichia coli promoter DNA sequences.大肠杆菌启动子DNA序列的汇编与分析
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Distribution of uidA gene sequences in Escherichia coli isolates in water sources and comparison with the expression of beta-glucuronidase activity in 4-methylumbelliferyl-beta-D-glucuronide media.水源中大肠杆菌分离株uidA基因序列的分布以及与4-甲基伞形酮基-β-D-葡萄糖醛酸苷培养基中β-葡萄糖醛酸酶活性表达的比较。
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beta-Glucuronidase from Escherichia coli as a gene-fusion marker.来自大肠杆菌的β-葡萄糖醛酸酶作为一种基因融合标记物。
Proc Natl Acad Sci U S A. 1986 Nov;83(22):8447-51. doi: 10.1073/pnas.83.22.8447.
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Plasmids with the uidA reporter gene for the detection of promoters and transcription signals.带有uidA报告基因的质粒，用于检测启动子和转录信号。
Mol Gen Genet. 1988 May;212(2):390-2. doi: 10.1007/BF00334714.
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Transcriptional and translational signals of the uidA gene in Escherichia coli K12.大肠杆菌K12中uidA基因的转录和翻译信号
Mol Gen Genet. 1987 Jul;208(3):490-8. doi: 10.1007/BF00328145.
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Linkage map of Escherichia coli K-12, edition 8.大肠杆菌K-12连锁图谱，第8版。
Microbiol Rev. 1990 Jun;54(2):130-97. doi: 10.1128/mr.54.2.130-197.1990.
9
Detection of Escherichia coli and Shigella spp. in water by using the polymerase chain reaction and gene probes for uid.利用聚合酶链反应和uid基因探针检测水中的大肠杆菌和志贺氏菌属。
Appl Environ Microbiol. 1991 Apr;57(4):1013-7. doi: 10.1128/aem.57.4.1013-1017.1991.
10
Identification of uidA gene sequences in beta-D-glucuronidase-negative Escherichia coli.在β-D-葡萄糖醛酸酶阴性大肠杆菌中鉴定uidA基因序列。
Appl Environ Microbiol. 1991 Jan;57(1):320-3. doi: 10.1128/aem.57.1.320-323.1991.
Nucleic Acids Res. 1983 Apr 25;11(8):2237-55. doi: 10.1093/nar/11.8.2237.
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Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis.利用寡脱氧核苷酸定向诱变构建改良的M13载体。
Gene. 1983 Dec;26(1):101-6. doi: 10.1016/0378-1119(83)90040-9.
5
Segment-specific mutagenesis of the regulatory region in the Escherichia coli galactose operon: isolation of mutations reducing the initiation of transcription and translation.大肠杆菌半乳糖操纵子调控区的区段特异性诱变：降低转录和翻译起始的突变的分离
Gene. 1983 Jan-Feb;21(1-2):121-31. doi: 10.1016/0378-1119(83)90154-3.
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pEMBL: a new family of single stranded plasmids.pEMBL：一类新型单链质粒。
Nucleic Acids Res. 1983 Mar 25;11(6):1645-55. doi: 10.1093/nar/11.6.1645.
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A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.一对用于选择双酶切限制片段任一条DNA链的新型M13载体。
Gene. 1982 Oct;19(3):269-76. doi: 10.1016/0378-1119(82)90016-6.
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Use of in vitro gene fusions to study the uxuR regulatory gene in Escherichia coli K-12: direction of transcription and regulation of its expression.利用体外基因融合技术研究大肠杆菌K-12中的uxuR调控基因：转录方向及其表达调控
J Bacteriol. 1982 Jun;150(3):1040-7. doi: 10.1128/jb.150.3.1040-1047.1982.
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Cloning and endonuclease restriction analysis of uidA and uidR genes in Escherichia coli K-12: determination of transcription direction for the uidA gene.大肠杆菌K-12中uidA和uidR基因的克隆及核酸内切酶限制分析：uidA基因转录方向的确定
J Bacteriol. 1982 Feb;149(2):587-94. doi: 10.1128/jb.149.2.587-594.1982.
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Homologies between different procaryotic DNA-binding regulatory proteins and between their sites of action.不同原核生物DNA结合调节蛋白之间及其作用位点之间的同源性。
EMBO J. 1982;1(5):591-5. doi: 10.1002/j.1460-2075.1982.tb01213.x.