Institute of Cell Biophysics, Russian Academy of Sciences, Federal Research Center Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Pushchino, Moscow region, Russia.
Bull Exp Biol Med. 2024 Apr;176(6):816-819. doi: 10.1007/s10517-024-06115-x. Epub 2024 Jun 19.
We studied the effect of antibiotic gentamicin at concentrations of 0.05, 0.1, 0.2, 0.4, and 1 mg/ml on the maintenance of sperm motility of the common toad Bufo bufo during cold storage of spermic urine samples at 4°C. Parameters of sperm motility during storage of samples with gentamicin at concentrations of 0.05-0.4 mg/ml did not differ significantly, but were higher (p<0.0001) than in the control (storage without antibiotic). Gentamicin at a concentration of 1 mg/ml had a negative effect on sperm motility. After 2 weeks of storage of toad spermic urine samples with gentamicin, the largest number of sperm was preserved when using antibiotic at a concentration of 0.4 mg/ml.
我们研究了浓度为 0.05、0.1、0.2、0.4 和 1mg/ml 的抗生素庆大霉素对蟾蜍 Bufo bufo 精子活力的维持作用，这些精子在 4°C 的低温下保存在精子尿液样本中。在浓度为 0.05-0.4mg/ml 的庆大霉素存在的情况下，样本储存期间精子运动的参数没有显著差异，但明显高于对照组（无抗生素储存）（p<0.0001）。浓度为 1mg/ml 的庆大霉素对精子活力有负面影响。在 2 周的蟾蜍精子尿液样本储存中，当使用浓度为 0.4mg/ml 的抗生素时，保留的精子数量最多。
