豚鼠纹状体中一种31千道尔顿假定脑啡肽前体的部分纯化及特性分析
Partial purification and characterisation of a 31 kDalton putative enkephalin precursor from guinea pig striatum.
作者信息
Metters K M, Beaumont A, Rossier J, Hughes J
出版信息
Neuropeptides. 1985 Feb;5(4-6):505-8. doi: 10.1016/0143-4179(85)90065-4.
The presence of several putative precursors for Met-enkephalin (ME) and Leu-enkephalin (LE) has been demonstrated in guinea pig striatal extracts. The major high molecular weight (Mr) species (31000) contained 9% of the total ME-immunoreactivity (IR) after digest. This species has been partially purified and characterized using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and nitrocellulose blotting techniques as well as highly specific radioimmunoassay (RIA) for ME, LE and ME-Arg6-Phe7.
在豚鼠纹状体提取物中已证实存在几种甲硫氨酸脑啡肽（ME）和亮氨酸脑啡肽（LE）的假定前体。主要的高分子量（Mr）物种（31000）在消化后含有总ME免疫反应性（IR）的9%。已使用十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）、硝酸纤维素印迹技术以及针对ME、LE和ME-Arg6-Phe7的高度特异性放射免疫测定（RIA）对该物种进行了部分纯化和表征。
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