Akrami Sousan, Ekrami Alireza, Avarvand Arshid Y
Department of Microbiology, School of Medicine Tehran University of Medical Sciences Tehran Iran.
Department of Laboratory Sciences, School of Allied Medical Sciences Ahvaz Jundishapur University of Medical Sciences Ahvaz Iran.
Health Sci Rep. 2024 Jun 19;7(6):e2138. doi: 10.1002/hsr2.2138. eCollection 2024 Jun.
Multidrug and extensive drug-resistant was extracted from burn patients referring to burn centers in southwest Iran so that biofilm generation and antibiotic resistance could be investigated.
A specific primer was used to confirm all our considered 110 culture-positive reports on 345 burn patients. The resistance of to seven antibiotics and Colistin with minimum inhibitory concentration (MIC) was assessed. Biofilm formation was assessed by the phenotypic study of specimens under Congo red agar and microtiter plate assays.
One hundred and 10 clinical isolates taken from burn wound infections were validated. Among isolates, Piperacillin, Ceftazidime, Maeropenem, Gentamycin, and Gatifloacin had the highest resistance to antibiotics, while Ticarcillin-Clavulanic acid and Ceftolozane-Tazobactam showed the least resistance. MICs were then evaluated via the E test. Seven isolates were resistant to colistin. Colistin reference MICs for multidrug-resistant prevalence was 38%, while it was 22% for extensively drug-resistant (XDR) . One was pandrug-resistant (PDR). Under Congo red agar test, 66 isolates (67%) formed biofilms and black colonies, whereas 44 isolates (50%) had red colonies. In MTP, 76% formed biofilm. 40%, 32%, 21% of the isolates were strong, moderate, and weak biofilm formers, respectively, while 43% did not form biofilms.
The resistance to antimicrobial agents has largely challenged the control of the infection. Accordingly, a higher resistance occurred when the isolates were transferred to the patients. Less than 50% samples generated strong biofilms. Consequently, hygienic measurements are essential to inhibit transmission to hospitalized patients.
从伊朗西南部烧伤中心转诊的烧伤患者中分离出多重耐药和广泛耐药菌，以研究生物膜形成和抗生素耐药性。
使用特异性引物对我们所关注的345例烧伤患者的110份培养阳性报告进行确认。评估分离菌对七种抗生素和黏菌素的最低抑菌浓度（MIC）耐药性。通过刚果红琼脂平板和微量滴定板试验对标本进行表型研究来评估生物膜形成情况。
从烧伤创面感染中分离出的110株临床菌株得到验证。在分离菌中，哌拉西林、头孢他啶、美罗培南、庆大霉素和加替沙星对抗生素的耐药性最高，而替卡西林-克拉维酸和头孢洛扎-他唑巴坦的耐药性最低。然后通过E试验评估MIC。七株分离菌对黏菌素耐药。多重耐药菌中黏菌素参考MIC的流行率为38%，而广泛耐药菌（XDR）为22%。一株为泛耐药菌（PDR）。在刚果红琼脂试验中，66株分离菌（67%）形成生物膜并产生黑色菌落，而44株分离菌（50%）有红色菌落。在微量滴定板试验中，76%形成生物膜。分离菌中分别有40%、32%、21%为强、中、弱生物膜形成菌，而43%不形成生物膜。
分离菌对抗菌药物耐药性在很大程度上对感染控制构成挑战。因此，当分离菌传播给患者时耐药性更高。不到50%的样本产生强生物膜。因此，卫生措施对于抑制分离菌传播给住院患者至关重要。
