Mirzaei Bahman, Bazgir Zahra Norouzi, Goli Hamid Reza, Iranpour Fatemeh, Mohammadi Fatemeh, Babaei Ryhaneh
Department of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Science, Sari, Iran.
Department of Medical Microbiology and Virology, School of Medicine, Zanjan University of Medical Science, Zanjan, Iran.
BMC Res Notes. 2020 Aug 10;13(1):380. doi: 10.1186/s13104-020-05224-w.
Multi and extensively drug-resistant (MDR and XDR), Pseudomonas aeruginosa (P. aeruginosa) and Acinetobacter baumannii (A. baumannii) are two main causative agents of nosocomial infections leading to increased morbidity and mortality. We aim to study the prevalence of MDR and XDR-A. baumannii and P. aeruginosa phenotypes in clinical specimens. We conducted this for 1 year (2017-2018) and isolated bacteria from the clinical samples. Then, XDR and MDR strains were determined by susceptibility testing (disc diffusion).
Out of 3248 clinical samples, A. baumannii and P. aeruginosa strains were detected in 309(9.51%) of them. Susceptibility testing indicated that (16.50%) and (15.53%) of the P. aeruginosa and (74.75%) and (73.13%) of the A. baumannii isolates were screened as the MDR and XDR strains. The frequency of MDR isolates was higher in wound samples 222 (71.8%). This rate in behavioral intensive care unit (BICU) and restoration ward, were 187 (60.5%) and 63 (20.4%). The frequency of XDR isolates in BICU 187 (59.54%), restoration 58(18.77%), and burns 30 (9.70%) were assessed as well. Considering high isolation rates of MDR and XDR of mentioned strains, it is necessary to apply prevention criteria for eradication of the mentioned bacteria from hospital wards.
多重耐药和广泛耐药的铜绿假单胞菌及鲍曼不动杆菌是医院感染的两种主要病原体，会导致发病率和死亡率上升。我们旨在研究临床标本中多重耐药和广泛耐药鲍曼不动杆菌及铜绿假单胞菌表型的流行情况。我们进行了为期1年（2017 - 2018年）的研究，从临床样本中分离细菌。然后，通过药敏试验（纸片扩散法）确定广泛耐药和多重耐药菌株。
在3248份临床样本中，检测到309份（9.51%）含有鲍曼不动杆菌和铜绿假单胞菌菌株。药敏试验表明，铜绿假单胞菌分离株中有16.50%和15.53%被筛选为多重耐药和广泛耐药菌株，鲍曼不动杆菌分离株中有74.75%和73.13%被筛选为多重耐药和广泛耐药菌株。伤口样本中多重耐药分离株的频率较高，为222份（71.8%）。行为重症监护病房（BICU）和康复病房的这一比例分别为187份（60.5%）和63份（20.4%）。还评估了BICU中广泛耐药分离株的频率为187份（59.54%），康复病房为58份（18.77%），烧伤病房为30份（9.70%）。考虑到上述菌株多重耐药和广泛耐药的高分离率，有必要应用预防标准以消除医院病房中的上述细菌。
