Bio-photonics and Green-photonics Laboratory, Department of Physics, Indian Institute of Technology Delhi, New Delhi, India.
Department of Physics and Technology, UiT The Arctic University of Norway, Tromsø, Norway.
J Biophotonics. 2024 Aug;17(8):e202400088. doi: 10.1002/jbio.202400088. Epub 2024 Jun 20.
Hyperspectral quantitative phase microscopy (HS-QPM) involves the acquisition of phase images across narrow spectral bands, which enables wavelength-dependent study of different biological samples. In the present work, a compact Linnik-type HS-QPM system is developed to reduce the instability and complexity associated with conventional HS-QPM techniques. The use of a single objective lens for both reference and sample arms makes the setup compact. The capabilities of the system are demonstrated by evaluating the HS phase map of both industrial and biological specimens. Phase maps of exfoliated cheek cells at different wavelengths are stacked to form a HS phase cube, adding spectral dimensionality to spatial phase distribution. Analysis of wavelength response of different cellular components are performed using principal component analysis to identify dominant spectral features present in the HS phase dataset. Findings of the study emphasize on the efficiency and effectiveness of HS-QPM for advancing cellular characterization in biomedical research and clinical applications.
高光谱定量相显微镜（HS-QPM）涉及在窄光谱带中获取相位图像，这使得能够对不同的生物样本进行波长相关的研究。在本工作中，开发了一种紧凑的 Linnik 型 HS-QPM 系统，以降低与传统 HS-QPM 技术相关的不稳定性和复杂性。参考臂和样品臂均使用单个物镜，使设置更加紧凑。通过评估工业和生物标本的 HS 相位图来证明系统的性能。将不同波长下的剥落颊细胞的相位图堆叠以形成 HS 相位立方体，从而为空间相位分布增加了光谱维。使用主成分分析对不同细胞成分的波长响应进行分析，以识别 HS 相位数据集中存在的主要光谱特征。该研究的结果强调了 HS-QPM 在推进生物医学研究和临床应用中的细胞特征分析方面的效率和有效性。
