Atta Supriya, Vo-Dinh Tuan
Fitzpatrick Institute for Photonics, Durham, NC, 27708, USA; Department of Biomedical Engineering, Duke University, Durham, NC, 27708, USA.
Fitzpatrick Institute for Photonics, Durham, NC, 27708, USA; Department of Biomedical Engineering, Duke University, Durham, NC, 27708, USA; Department of Chemistry, Duke University, Durham, NC, 27708, USA.
Talanta. 2024 Oct 1;278:126373. doi: 10.1016/j.talanta.2024.126373. Epub 2024 Jun 6.
Recently, solution-based surface-enhanced Raman scattering (SERS) detection technique has been widely recognized due to its cost-effectiveness, simplicity, and ease of use. However, solution-based SERS is limited for practical applications mainly because of the weak adsorption affinity of the target biomolecules to the surface of plasmonic nanoparticles. Herein, we developed a highly sensitive solution-based SERS sensing platform based on mercaptopropionic acid (MPA)-capped silver-coated gold nanostars (SGNS@MPA), which allows efficient enrichment on the nanostars surface for improved detection of an analyte: creatinine, a potential biomarker of chronic kidney disease (CKD). The SGNS@MPA exhibited high enrichment ability towards creatinine molecules in alkaline medium (pH-9) through multiple hydrogen bonding interaction, which causes aggregation of the nanoparticles and enhances the SERS signal of creatinine. The detection limit for creatinine was achieved at 0.1 nM, with a limit of detection (LOD) value of 14.6 pM. As a proof-of-concept demonstration, we conducted the first quantitative detection of creatinine in noninvasive human fluids, such as saliva and sweat, under separation-free conditions. We achieved a detection limit of up to 1 nM for both saliva and sweat, with LOD values as low as 0.136 nM for saliva and 0.266 nM for sweat. Overall, our molecular enrichment strategy offers a new way to improve the solution-based SERS detection technique for real-world practical applications in point-of-care settings and low-resource settings.
最近，基于溶液的表面增强拉曼散射（SERS）检测技术因其成本效益高、操作简单且易于使用而得到广泛认可。然而，基于溶液的SERS在实际应用中受到限制，主要是因为目标生物分子与等离子体纳米颗粒表面的吸附亲和力较弱。在此，我们开发了一种基于巯基丙酸（MPA）包覆的银包金纳米星（SGNS@MPA）的高灵敏度基于溶液的SERS传感平台，该平台允许在纳米星表面进行高效富集，以改进对一种分析物——肌酐（慢性肾病（CKD）的潜在生物标志物）的检测。SGNS@MPA在碱性介质（pH = 9）中通过多重氢键相互作用对肌酐分子表现出高富集能力，这导致纳米颗粒聚集并增强了肌酐的SERS信号。肌酐的检测限达到0.1 nM，检测限（LOD）值为14.6 pM。作为概念验证演示，我们在无分离条件下对非侵入性人体体液（如唾液和汗液）中的肌酐进行了首次定量检测。我们对唾液和汗液的检测限均高达1 nM，唾液的LOD值低至0.136 nM，汗液的LOD值低至0.266 nM。总体而言，我们的分子富集策略为改进基于溶液的SERS检测技术提供了一种新方法，可用于即时护理和资源匮乏环境中的实际应用。
