Chang Shih-Hsin, Ko Yun-Fei, Liau Jian-Ching, Wu Cheng-Yeu, Hwang Tsong-Long, Ojcius David M, Young John D, Martel Jan
Center for Molecular and Clinical Immunology, Chang Gung University, Taoyuan, Taiwan; Chang Gung Immunology Consortium, Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan.
Chang Gung Immunology Consortium, Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan; Chang Gung Biotechnology Corporation, Taipei, Taiwan; Biochemical Engineering Research Center, Ming Chi University of Technology, New Taipei City, Taiwan.
Biomed J. 2024 Jun 18;48(2):100754. doi: 10.1016/j.bj.2024.100754.
The incidence of autoimmune diseases is increasing in developed countries, possibly due to the modern Western diet and lifestyle. We showed earlier that polysaccharides derived from the medicinal fungus Hirsutella sinensis produced anti-inflammatory, anti-diabetic and anti-obesity effects by modulating the gut microbiota and increasing the abundance of the commensal Parabacteroides goldsteinii in mice fed with a high-fat diet.
We examined the effects of the prebiotics, H. sinensis polysaccharides, and probiotic, P. goldsteinii, in a mouse model of imiquimod-induced systemic lupus erythematosus.
The fungal polysaccharides and P. goldsteinii reduced markers of lupus severity, including the increase of spleen weight, proteinuria, and serum levels of anti-DNA auto-antibodies and signal transducer and activator of transcription 4 (STAT4). Moreover, the polysaccharides and P. goldsteinii improved markers of kidney and liver functions such as creatinine, blood urea nitrogen, glomerulus damage and fibrosis, and serum liver enzymes. However, the prebiotics and probiotics did not produce consistent improvements in gut microbiota composition, colonic histology, or expression of tight junction proteins in colon tissues.
Our results indicate that H. sinensis polysaccharides and the probiotic P. goldsteinii can reduce lupus markers in imiquimod-treated mice. These prebiotics and probiotics may therefore be added to other interventions conducive of a healthy lifestyle in order to counter autoimmune diseases.
在发达国家，自身免疫性疾病的发病率正在上升，这可能归因于现代西方饮食和生活方式。我们之前的研究表明，从药用真菌中华被毛孢中提取的多糖通过调节肠道微生物群以及增加高脂饮食喂养小鼠体内共生的戈氏副拟杆菌丰度，产生抗炎、抗糖尿病和抗肥胖作用。
我们在咪喹莫特诱导的系统性红斑狼疮小鼠模型中研究了益生元（中华被毛孢多糖）和益生菌（戈氏副拟杆菌）的作用。
真菌多糖和戈氏副拟杆菌降低了狼疮严重程度指标，包括脾脏重量增加、蛋白尿、抗DNA自身抗体血清水平以及信号转导和转录激活因子4（STAT4）。此外，多糖和戈氏副拟杆菌改善了肾功能和肝功能指标，如肌酐、血尿素氮、肾小球损伤和纤维化以及血清肝酶。然而，益生元和益生菌在肠道微生物群组成、结肠组织学或结肠组织紧密连接蛋白表达方面并未产生一致的改善效果。
我们的结果表明，中华被毛孢多糖和益生菌戈氏副拟杆菌可降低咪喹莫特处理小鼠的狼疮指标。因此，这些益生元和益生菌可能会被添加到其他有利于健康生活方式的干预措施中，以对抗自身免疫性疾病。
