Ginting Binawati, Yahya Mustanir, Saidi Nurdin, Maulana Ilham, Murniana Murniana, Safitri Eka, Bahi Muhammad, Rosiyana Yetty, Novani Hazrina, Milza Devia Nurul Azla
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh, Indonesia.
J Adv Pharm Technol Res. 2024 Apr-Jun;15(2):70-74. doi: 10.4103/JAPTR.JAPTR_470_23. Epub 2024 May 6.
Leaves of have medicinal potential which has gained attention from researchers around the world. This study has an objective to screen the antioxidant and cytotoxicity of ethyl acetate extract from leaves and its fraction. The fine powder of was macerated in methanol and further partitioned using two different solvents, namely n-hexane and ethyl acetate. In this article, we reported the screening results for ethyl acetate extract. Fractionation was then performed on the extract by means of column chromatography by gradient elution resulting in five combined fractions. Brine shrimp lethality test and 1-diphenyl-2-pycrilhidrazil (DPPH) assays were employed to evaluate the cytotoxicity and antioxidant of the extract, respectively. Characterization using gas chromatography-mass spectroscopy (GC-MS) was then conducted. The cytotoxicity of the samples was indicated by median lethal concentration values ranging from 28.84 to 1023.3 ppm. As for the antioxidant activity, the DPPH median inhibitory concentration values ranged from 4.12 to 180.66 ppm. GC-MS analysis on the most bioactive fraction revealed the predominating phytochemical contents of neophytadiene, palmitic acid, and phytol. In conclusion, the fraction of ethyl acetate extract from leaves could potentially act as a strong antioxidant and moderate cytotoxic agent.
[植物名称]的叶子具有药用潜力，已引起世界各地研究人员的关注。本研究旨在筛选[植物名称]叶子的乙酸乙酯提取物及其馏分的抗氧化和细胞毒性。将[植物名称]的细粉在甲醇中浸渍，然后用两种不同的溶剂，即正己烷和乙酸乙酯进一步分离。在本文中，我们报告了乙酸乙酯提取物的筛选结果。然后通过梯度洗脱柱色谱法对提取物进行分馏，得到五个合并馏分。分别采用卤虫致死试验和1,1-二苯基-2-苦基肼（DPPH）测定法评估提取物的细胞毒性和抗氧化活性。然后进行气相色谱-质谱联用（GC-MS）表征。样品的细胞毒性通过中位致死浓度值在28.84至1023.3 ppm之间表示。至于抗氧化活性，DPPH中位抑制浓度值在4.12至180.66 ppm之间。对生物活性最强的馏分进行的GC-MS分析揭示了新植二烯、棕榈酸和叶绿醇的主要植物化学成分。总之，[植物名称]叶子的乙酸乙酯提取物馏分可能具有强大的抗氧化剂和中度细胞毒性剂的作用。
