Department of Clinical Laboratory, The Second Hospital of Jilin University, Changchun, Jilin 130000, People's Republic of China.
Specialty in Pharmaceutical Analysis, College of Pharmacy, Beihua University, Jilin, Jilin 132013, People's Republic of China.
Biosci Rep. 2024 Jul 31;44(7). doi: 10.1042/BSR20232068.
Learning and memory impairment (LMI), a common degenerative central nervous system disease. Recently, more and more studies have shown that Ganoderma lucidum (GL) can improve the symptoms of LMI. The active ingredients in GL and their corresponding targets were screened through TCMSP (Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform) and BATMAN-TCM (Bioinformatics Analysis Tool for Molecular Mechanism of Traditional Chinese Medicine) databases, and the potential LMI targets were searched for through GeneCard (GeneCards Human Gene Database) and DrugBank. Then, we construct a 'main active ingredient-target' network and a protein-protein interaction (PPI) network diagram.The GO (Gene Ontology) functional enrichment analysis and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway annotation analysis were performed on the common targets through DAVID (Database for Annotation Visualization and Integrated Discovery) to clarify the potential molecular mechanism of action of active ingredients in GL. The tumor necrosis factor (TNF) protein was verified by Western blot; Twenty one active ingredients in GL and 142 corresponding targets were screened out, including 59 targets shared with LMI. The 448 biological processes shown by the GO functional annotation results and 55 signal pathways shown by KEGG enrichment analysis were related to the improvement of LMI by GL, among which the correlation of Alzheimer's disease pathway is the highest, and TNF was the most important protein; TNF can improve LMI. GL can improve LMI mainly by 10 active ingredients in it, and they may play a role by regulating Alzheimer's disease pathway and TNF protein.
学习和记忆障碍（LMI），一种常见的退行性中枢神经系统疾病。最近，越来越多的研究表明灵芝（GL）可以改善 LMI 症状。通过 TCMSP（中药系统药理学数据库和分析平台）和 BATMAN-TCM（中药分子机制生物信息学分析工具）数据库筛选 GL 的活性成分及其相应靶点，并通过 GeneCard（人类基因数据库）和 DrugBank 搜索潜在的 LMI 靶点。然后，我们构建了一个“主要活性成分-靶点”网络和一个蛋白质-蛋白质相互作用（PPI）网络图。通过 DAVID（基因注释可视化和综合发现数据库）对共同靶点进行 GO（基因本体论）功能富集分析和 KEGG（京都基因与基因组百科全书）通路注释分析，以阐明 GL 中活性成分的潜在作用机制。通过 Western blot 验证肿瘤坏死因子（TNF）蛋白；从 GL 中筛选出 21 种活性成分和 142 个相应靶点，其中 59 个靶点与 LMI 共享。GO 功能注释结果显示的 448 个生物学过程和 KEGG 富集分析显示的 55 个信号通路与 GL 改善 LMI 有关，其中阿尔茨海默病途径的相关性最高，TNF 是最重要的蛋白；TNF 可以改善 LMI。GL 主要通过其 10 种活性成分改善 LMI，它们可能通过调节阿尔茨海默病途径和 TNF 蛋白发挥作用。
