一种从地表水中回收小肠结肠炎耶尔森菌的简化方法的应用。
Application of a simplified method for recovery of Yersinia enterocolitica from surface waters.
作者信息
Marinelli G, D'Innocenzo C, Fabiani L, Leoni V
出版信息
Appl Environ Microbiol. 1985 May;49(5):1348-9. doi: 10.1128/aem.49.5.1348-1349.1985.
Abstract
Yersinia enterocolitica was recovered from surface water by a simplified method: samples were taken by means of the Moore tampon and, after a short, warm preincubation in peptone-sorbitol-bile salts broth, were quickly passed to an alkali solution. Immediately after this, samples were directly plated on MacConkey agar. Y. enterocolitica was isolated from 60% of the water samples, and the identification confirmation of the strains chosen was 100%.
摘要
通过一种简化方法从地表水中分离出小肠结肠炎耶尔森菌
使用穆尔棉塞采集样本，在蛋白胨 - 山梨醇 - 胆盐肉汤中进行短暂的温热预培养后，迅速转移至碱性溶液中。在此之后，样本立即直接接种于麦康凯琼脂平板上。从60%的水样中分离出了小肠结肠炎耶尔森菌，所选菌株的鉴定确认率为100%。
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