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一种从地表水中回收小肠结肠炎耶尔森菌的简化方法的应用。

Application of a simplified method for recovery of Yersinia enterocolitica from surface waters.

作者信息

Marinelli G, D'Innocenzo C, Fabiani L, Leoni V

出版信息

Appl Environ Microbiol. 1985 May;49(5):1348-9. doi: 10.1128/aem.49.5.1348-1349.1985.

Abstract

Yersinia enterocolitica was recovered from surface water by a simplified method: samples were taken by means of the Moore tampon and, after a short, warm preincubation in peptone-sorbitol-bile salts broth, were quickly passed to an alkali solution. Immediately after this, samples were directly plated on MacConkey agar. Y. enterocolitica was isolated from 60% of the water samples, and the identification confirmation of the strains chosen was 100%.

摘要

通过一种简化方法从地表水中分离出小肠结肠炎耶尔森菌

使用穆尔棉塞采集样本,在蛋白胨 - 山梨醇 - 胆盐肉汤中进行短暂的温热预培养后,迅速转移至碱性溶液中。在此之后,样本立即直接接种于麦康凯琼脂平板上。从60%的水样中分离出了小肠结肠炎耶尔森菌,所选菌株的鉴定确认率为100%。

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