用于从非食品来源分离小肠结肠炎耶尔森菌的改良富集肉汤。
Modified enrichment broth for isolation of Yersinia enterocolitica from nonfood sources.
作者信息
Weagant S D, Kaysner C A
出版信息
Appl Environ Microbiol. 1983 Feb;45(2):468-71. doi: 10.1128/aem.45.2.468-471.1983.
Abstract
Sorbitol-bile salts broth was modified by the addition of 0.5% peptone for the enrichment of Yersinia enterocolitica in nonfood samples. A quantitative comparison was made of the growth of Y. enterocolitica in sorbitol-bile salts broth and peptone-sorbitol-bile salts broth at 22 degrees C. Incubation for 48 h resulted in an average 7.79 log10 increase in a viable cell number for six serotypes with peptone-sorbitol-bile salts broth compared with an average 1.85 log10 increase for sorbitol-bile salts broth. Recovery from seeded seawater showed a greater than 100-fold increase in sensitivity with peptone-sorbitol-bile salts broth enrichment. Isolation of indigenous Y. enterocolitica from fresh and marine waters was also significantly enhanced.
摘要
通过添加0.5%蛋白胨对山梨醇-胆盐肉汤进行改良，用于在非食品样本中富集小肠结肠炎耶尔森菌。对小肠结肠炎耶尔森菌在山梨醇-胆盐肉汤和蛋白胨-山梨醇-胆盐肉汤中于22℃下的生长情况进行了定量比较。培养48小时后，与山梨醇-胆盐肉汤中活菌数平均增加1.85个对数单位相比，蛋白胨-山梨醇-胆盐肉汤中6个血清型的活菌数平均增加7.79个对数单位。从接种的海水中回收显示，使用蛋白胨-山梨醇-胆盐肉汤富集时灵敏度提高了100倍以上。从淡水和海水中分离本土小肠结肠炎耶尔森菌的能力也显著增强。
相似文献
1
Modified enrichment broth for isolation of Yersinia enterocolitica from nonfood sources.用于从非食品来源分离小肠结肠炎耶尔森菌的改良富集肉汤。
Appl Environ Microbiol. 1983 Feb;45(2):468-71. doi: 10.1128/aem.45.2.468-471.1983.
2
Application of a simplified method for recovery of Yersinia enterocolitica from surface waters.一种从地表水中回收小肠结肠炎耶尔森菌的简化方法的应用。
Appl Environ Microbiol. 1985 May;49(5):1348-9. doi: 10.1128/aem.49.5.1348-1349.1985.
3
[Comparison between two enrichment media for "Yersinia enterocolitica" isolation].["两种用于分离小肠结肠炎耶尔森菌的增菌培养基的比较"]
Quad Sclavo Diagn. 1981 Dec;17(4):521-4.
4
Alkalotolerance of Yersinia enterocolitica as a basis for selective isolation from food enrichments.小肠结肠炎耶尔森菌的耐碱性作为从食品增菌培养物中进行选择性分离的基础。
Appl Environ Microbiol. 1983 Jul;46(1):22-7. doi: 10.1128/aem.46.1.22-27.1983.
5
Isolation of Yersinia enterocolitica from foods.从食品中分离出小肠结肠炎耶尔森菌。
Int J Food Microbiol. 1992 Oct;17(2):75-84. doi: 10.1016/0168-1605(92)90107-e.
6
Isolation of Yersinia enterocolitica and related species from porcine samples obtained from an abattoir.从屠宰场获取的猪样本中分离小肠结肠炎耶尔森菌及相关菌种。
J Appl Bacteriol. 1984 Jun;56(3):421-7. doi: 10.1111/j.1365-2672.1984.tb01370.x.
7
[Various isolation and enrichment media for isolating Yersinia enterocolitica].
Arch Inst Pasteur Alger. 1992;58:27-39.
8
Yersinia enterocolitica in slaughter pig tonsils: enumeration and detection by enrichment versus direct plating culture.肠侵袭性大肠埃希菌在屠宰猪扁桃体中的计数和检测：富集培养与直接平板培养的比较。
Food Microbiol. 2010 Feb;27(1):158-61. doi: 10.1016/j.fm.2009.09.011. Epub 2009 Sep 27.
9
Effect of sampling and short isolation methodologies on the recovery of human pathogenic Yersinia enterocolitica from pig tonsils.采样和短期隔离方法对猪扁桃体中人畜共患病耶尔森氏菌回收率的影响。
Foodborne Pathog Dis. 2012 Jul;9(7):600-6. doi: 10.1089/fpd.2012.1128. Epub 2012 Jun 12.
10
Identification of Yersinia enterocolitica in minced meat: a comparative analysis of API 20E, Yersinia identification kit and a 16S rRNA-based PCR method.碎肉中肠炎耶尔森菌的鉴定：API 20E、耶尔森菌鉴定试剂盒和基于16S rRNA的PCR方法的比较分析
J Vet Med B Infect Dis Vet Public Health. 2004 Feb;51(1):23-7. doi: 10.1046/j.1439-0450.2003.00718.x.
引用本文的文献
1
Evaluation of a single procedure allowing the isolation of enteropathogenic Yersinia along with other bacterial enteropathogens from human stools.一种从人类粪便中分离出肠致病性耶尔森菌和其他细菌病原体的单一方法的评估。
PLoS One. 2012;7(7):e41176. doi: 10.1371/journal.pone.0041176. Epub 2012 Jul 20.
2
Medium for presumptive identification of Yersinia enterocolitica.用于耶尔森氏小肠结肠炎杆菌初步鉴定的培养基。
Appl Environ Microbiol. 1983 Feb;45(2):472-3. doi: 10.1128/aem.45.2.472-473.1983.
3
Application of a simplified method for recovery of Yersinia enterocolitica from surface waters.一种从地表水中回收小肠结肠炎耶尔森菌的简化方法的应用。
Appl Environ Microbiol. 1985 May;49(5):1348-9. doi: 10.1128/aem.49.5.1348-1349.1985.
4
Recovery of Yersinia enterocolitica from patients with gastrointestinal disturbances. Lack of efficacy of alkali method.从胃肠功能紊乱患者中分离出小肠结肠炎耶尔森菌。碱法无效。
Eur J Epidemiol. 1987 Dec;3(4):448-50. doi: 10.1007/BF00145662.
本文引用的文献
1
Yersiniae in pond water and snails.池塘水和蜗牛中的耶尔森氏菌。
J Wildl Dis. 1976 Oct;12(4):492-6. doi: 10.7589/0090-3558-12.4.492.
2
Alkali method for rapid recovery of Yersinia enterocolitica and Yersinia pseudotuberculosis from foods.从食品中快速回收小肠结肠炎耶尔森菌和假结核耶尔森菌的碱法
Appl Environ Microbiol. 1980 Jan;39(1):135-40. doi: 10.1128/aem.39.1.135-140.1980.
3
Yersinia enterocolitica in drinking-water.饮用水中的小肠结肠炎耶尔森菌。
Scand J Infect Dis. 1972;4(2):125-7. doi: 10.3109/inf.1972.4.issue-2.11.
4
Survey on the incidence of Yersinia enterocolitica in the province of Ontario.安大略省小肠结肠炎耶尔森氏菌发病率调查。
Can J Public Health. 1973 Sep-Oct;64(5):477-87.
5
Yersinia enterocolitica septicemia. Source of infection and incubation period identified.小肠结肠炎耶尔森菌败血症。已确定感染源和潜伏期。
N Y State J Med. 1974 Nov;74(12):2226-30.
6
Yersinia enterocolitica in borwn trout (Salmo trutta L.) from Norway.来自挪威的褐鳟（Salmo trutta L.）中的小肠结肠炎耶尔森菌
Acta Pathol Microbiol Scand B. 1976 Feb;84(1):66-8. doi: 10.1111/j.1699-0463.1976.tb01903.x.
7
Occurrence of Yersinia enterocolitica in house rats.家鼠中肠炎耶尔森菌的存在情况。
Appl Environ Microbiol. 1978 Aug;36(2):314-8. doi: 10.1128/aem.36.2.314-318.1978.
8
Isolation of Yersinia enterocolitica from raw milk.从生牛奶中分离出小肠结肠炎耶尔森菌。
Appl Environ Microbiol. 1978 Jan;35(1):54-8. doi: 10.1128/aem.35.1.54-58.1978.
9
Microbiological methods. Problems in the recovery and identification of Yersinia from food.
J Assoc Off Anal Chem. 1978 Jul;61(4):761-771.
Zotero 插件