Mason J C, Venables P J, Smith P R, Maini R N
Ann Rheum Dis. 1985 May;44(5):287-93. doi: 10.1136/ard.44.5.287.
In order to examine the interactions between isolated rheumatoid factors (RFs) and cell nuclear antigens we have prepared 10 RFs by affinity chromatography against IgG coupled to Sepharose. Of these, seven cross reacted with cell nuclei on indirect immunofluorescence. The nuclear antigen appeared to be DNA histones by indirect immunofluorescence on rat liver, though on immunoblotting the rheumatoid factors also reacted with three non-histone polypeptides which were identified in the soluble fraction of nuclear extracts. We were unable to show any relationship between these polypeptides and rheumatoid arthritis nuclear antigen. These reactions represent a hitherto unrecognised phenomenon, which extends the range of antigens recognised by rheumatoid factors. We suggest that the immunopathogenic significance of RFs may not be restricted to their reactivity with IgG, and that non-histone nuclear proteins merit further investigation.
为了研究分离出的类风湿因子（RFs）与细胞核抗原之间的相互作用，我们通过与偶联到琼脂糖凝胶上的IgG进行亲和层析制备了10种RFs。其中，7种在间接免疫荧光中与细胞核发生交叉反应。通过对大鼠肝脏进行间接免疫荧光检测，核抗原似乎是DNA组蛋白，不过在免疫印迹中，类风湿因子还与三种非组蛋白多肽发生反应，这些多肽在核提取物的可溶部分中被鉴定出来。我们未能证明这些多肽与类风湿关节炎核抗原之间存在任何关系。这些反应代表了一种迄今未被认识的现象，这扩展了类风湿因子所识别的抗原范围。我们认为，RFs的免疫致病意义可能不限于它们与IgG的反应性，非组蛋白核蛋白值得进一步研究。
