Asymmetric Operations Sector, Applied Biological Sciences, The Johns Hopkins University Applied Physics Laboratory, Laurel, MD, USA.
Methods Mol Biol. 2024;2822:411-416. doi: 10.1007/978-1-0716-3918-4_25.
Various nanoparticle-based delivery systems have been developed for the encapsulation and protection of active cargoes. Lipid nanoparticles represent one of the most widely used nanoparticle-based delivery systems for in vitro and in vivo applications, especially for the delivery of ribonucleic acid (RNA). In this chapter, a simple bulk mixing method for the encapsulation of RNA is described along with characterization techniques for measuring encapsulation efficiency and nanoparticle physicochemical properties.
各种基于纳米粒子的递药系统已被开发出来，用于封装和保护活性载药。脂质纳米粒是最广泛应用于体外和体内应用的基于纳米粒子的递药系统之一，特别是用于递送核糖核酸（RNA）。本章描述了一种用于 RNA 包封的简单的批量混合方法，以及用于测量包封效率和纳米颗粒理化性质的表征技术。
