College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, China.
School of Medicine, Shihezi University, Shihezi, Xinjiang 832000, China.
Poult Sci. 2024 Aug;103(8):103954. doi: 10.1016/j.psj.2024.103954. Epub 2024 Jun 6.
Succinate has been shown to be a potentially beneficial nutritional supplement with a diverse range of physiological functions. However, it remains unknown whether succinate supplementation regulates lipid metabolism in chickens. The aim of this study was to explore how succinate affects fat deposition and the underlying mechanism involved in broilers and to determine the most appropriate level of succinate supplementation in the diet. A total of 640 one-day-old male yellow-feathered broilers were randomly divided into 4 groups with 8 replicates and 20 broilers per replicate. A basal diet was provided to the control group (CON). The experimental broilers were fed diets containing 0.2% (L), 0.4% (M), or 0.6% (H) succinate and the study was lasted for 21 d. The linear (l) and quadratic (q) effects of succinate addition were determined. The results indicated that supplementation with 0.4% succinate reduced ADFI, serum triglycerides (l, q; P < 0.05), glucose (q; P < 0.05), and increased high-density lipidprotein cholesterol (l, q; P < 0.05) concentrations in broilers. Moreover, 0.4% succinate affects lipid metabolism by decreasing the abdominal fat percentage and adipocyte surface area, the expression of genes that promote liposynthesis in the abdominal fat and liver, as well as increasing the expression of genes that promote lipolysis in the abdominal fat and liver. In addition, increased cecal propionic acid content (q, P < 0.05) was found in the M group compared to the CON group. The 16S rRNA sequence analysis showed that group M altered cecum microbial composition by increasing the abundance of genera such as Blautia and Sellimonas (P < 0.05). LC-MS metabolomic analysis revealed that the differential metabolites between the M and CON groups were enriched in amino acid-related pathways. In conclusion, the optimum level of succinate added to broiler diets in the present study was 0.4%. Succinate can potentially reduce fat accumulation in broilers by modulating the composition of the gut flora and amino acid metabolism related to lipid metabolism.
琥珀酸已被证明是一种具有多种生理功能的潜在有益营养补充剂。然而，琥珀酸补充剂是否能调节鸡的脂质代谢尚不清楚。本研究旨在探讨琥珀酸如何影响肉鸡的脂肪沉积及其潜在机制，并确定饲料中琥珀酸的最佳添加水平。
将 640 只 1 日龄雄性黄羽肉鸡随机分为 4 组，每组 8 个重复，每个重复 20 只。对照组（CON）饲喂基础日粮。实验组饲喂含 0.2%（L）、0.4%（M）或 0.6%（H）琥珀酸的日粮，试验持续 21d。确定了琥珀酸添加的线性（l）和二次（q）效应。结果表明，添加 0.4%琥珀酸可降低肉鸡的 ADFI、血清甘油三酯（l，q；P<0.05）、葡萄糖（q；P<0.05），并提高高密度脂蛋白胆固醇（l，q；P<0.05）浓度。
此外，0.4%琥珀酸通过降低腹部脂肪百分比和脂肪细胞表面积、降低腹部脂肪和肝脏中促进脂肪合成的基因表达、增加腹部脂肪和肝脏中促进脂肪分解的基因表达来影响脂质代谢。此外，与 CON 组相比，M 组盲肠丙酸含量增加（q，P<0.05）。16S rRNA 序列分析显示，M 组通过增加 Blautia 和 Sellimonas 等属的丰度改变盲肠微生物组成（P<0.05）。LC-MS 代谢组学分析表明，M 组与 CON 组之间的差异代谢物富集在与氨基酸代谢相关的途径中。
综上所述，本研究中肉鸡日粮中添加琥珀酸的最佳水平为 0.4%。琥珀酸通过调节与脂质代谢相关的肠道菌群组成和氨基酸代谢，可能减少肉鸡脂肪堆积。
