Funnell G R, Guinness M D
Antimicrob Agents Chemother. 1979 Sep;16(3):255-61. doi: 10.1128/AAC.16.3.255.
Autobac I, a recently introduced semiautomated method for rapid antibiotic susceptibility testing, has been evaluated by comparison with the calibrated dichotomous sensitivity disk diffusion technique, which is routinely used in many Australian hospitals. Only the most common clinical isolates, Staphylococcus aureus, Escherichia coli, Klebsiella sp., and Proteus mirabilis, were included in this evaluation, and an overall interpretive agreement of 93% was obtained. However, an unusually high rate of discrepancy was noted in several organism-antibiotic combinations, in particular E. coli and P. mirabilis with ampicillin, S. aureus with penicillin, and methicillin-resistant S. aureus with methicillin, erythromycin, and clindamycin. The discrepancies associated with ampicillin have been reduced from 29 and 24% for E. coli and P. mirabilis, respectively, to less than 5% after the utilization of commercial 10-micrograms diffusion disks, in preference to the lower antibiotic content disks supplied by the Autobac manufacturer. Furthermore, modifications in the interpretive procedure have eliminated discrepancies associated with S. aureus and penicillin.
Autobac I是最近推出的一种用于快速抗生素敏感性测试的半自动方法，已通过与校准二分敏感性纸片扩散技术进行比较来评估，该技术在许多澳大利亚医院中常规使用。本次评估仅纳入了最常见的临床分离株，即金黄色葡萄球菌、大肠杆菌、克雷伯菌属和奇异变形杆菌，总体解释一致性达到了93%。然而，在几种细菌 - 抗生素组合中发现了异常高的差异率，特别是大肠杆菌和奇异变形杆菌与氨苄西林、金黄色葡萄球菌与青霉素以及耐甲氧西林金黄色葡萄球菌与甲氧西林、红霉素和克林霉素的组合。在使用市售10微克扩散纸片而非Autobac制造商提供的抗生素含量较低的纸片后，与氨苄西林相关的差异率分别从大肠杆菌的29%和奇异变形杆菌的24%降至5%以下。此外，解释程序的修改消除了与金黄色葡萄球菌和青霉素相关的差异。
