Institute of Horticulture, Sichuan Academy of Agricultural Science, Chengdu, Sichuan, China.
Department of Technology Management, Sichuan Academy of Agricultural Science, Chengdu, Sichuan, China.
PLoS One. 2024 Jun 25;19(6):e0305867. doi: 10.1371/journal.pone.0305867. eCollection 2024.
Foliage color is considered an important ornamental character of Cymbidium tortisepalum (C. tortisepalum), which significantly improves its horticultural and economic value. However, little is understood on the formation mechanism underlying foliage-color variations.
In this study, we applied a multi-omics approach based on transcriptomics and metabolomics, to investigate the biomolecule mechanisms of metabolites changes in C. tortisepalum colour mutation cultivars.
A total of 508 genes were identified as differentially expressed genes (DEGs) between wild and foliage colour mutation C. tortisepalum cultivars based on transcriptomic data. KEGG enrichment of DEGs showed that genes involved in phenylalanine metabolism, phenylpropanoid biosynthesis, flavonoid biosynthesis and brassinosteroid biosynthesis were most significantly enriched. A total of 420 metabolites were identified in C. tortisepalum using UPLC-MS/MS-based approach and 115 metabolites differentially produced by the mutation cultivars were identified. KEGG enrichment indicated that the most metabolites differentially produced by the mutation cultivars were involved in glycerophospholipid metabolism, tryptophan metabolism, isoflavonoid biosynthesis, flavone and flavonol biosynthesis. Integrated analysis of the metabolomic and transcriptomic data showed that there were four significant enrichment pathways between the two cultivars, including phenylalanine metabolism, phenylpropanoid biosynthesis, flavone and flavonol biosynthesis and flavonoid biosynthesis.
The results of this study revealed the mechanism of metabolites changes in C. tortisepalum foliage colour mutation cultivars, which provides a new reference for breeders to improve the foliage color of C. tortisepalum.
叶片颜色被认为是春兰（Cymbidium tortisepalum）的一个重要观赏性状，显著提高了其园艺和经济价值。然而，叶片颜色变化的形成机制还知之甚少。
本研究采用基于转录组学和代谢组学的多组学方法，研究春兰花色突变品种中代谢物变化的生物分子机制。
基于转录组数据，共鉴定出 508 个差异表达基因（DEGs），这些基因在野生型和叶片颜色突变春兰品种之间存在差异。DEGs 的 KEGG 富集分析表明，参与苯丙氨酸代谢、苯丙烷生物合成、类黄酮生物合成和油菜素内酯生物合成的基因最为显著富集。采用 UPLC-MS/MS 方法共鉴定出春兰中 420 种代谢物，鉴定出突变品种差异产生的 115 种代谢物。KEGG 富集表明，突变品种差异产生的代谢物主要涉及甘油磷脂代谢、色氨酸代谢、异黄酮生物合成、黄酮和黄酮醇生物合成。代谢组学和转录组学数据的综合分析表明，两个品种之间存在四个显著富集途径，包括苯丙氨酸代谢、苯丙烷生物合成、黄酮和黄酮醇生物合成和类黄酮生物合成。
本研究结果揭示了春兰叶片颜色突变品种中代谢物变化的机制，为培育春兰叶片颜色提供了新的参考。
