Zhou Mi, Liu Yuncai, Wang Yucheng, Chang Yawen, Wu Qingjun, Gong Weirong, Du Yuzhou
Institute of Applied Entomology, College of Plant Protection, Yangzhou University, Yangzhou 225009, China.
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Insects. 2024 May 29;15(6):399. doi: 10.3390/insects15060399.
(Gennadius) is one of the most important invasive species in China, with strong insecticide resistance and thermotolerance. In this study, we investigated the effects of elevated temperature on the tolerance of MEMA1 to abamectin (AB) and thianethixam (TH) insecticides. We firstly cloned two new CYP450 genes from MEAM1, including one CYP6 family gene () and one CYP305 family gene (). The expression patterns of the two BtCYP450 genes were compared in response to high-temperature stress and insecticide exposure, and RNAi was then used to demonstrate the role that these two genes play in insecticide tolerance. The results showed that expression of the two BtCYP450 genes could be induced by exposure to elevated temperature or insecticide, but this gene expression could be inhibited to a certain extent when insects were exposed to the combined effects of high temperature and insecticide treatment. For AB treatment, the expression of the two BtCYP450 genes reached the lowest level when insects were exposed to a temperature of 41 °C and treated with AB (combined effects of temperature and insecticide). In contrast, TH treatment showed a general decrease in the expression of the two BtCYP450 genes with exposure to elevated temperatures. These findings suggest that insecticide tolerance in MEAM1 could be mediated by high temperatures. This study provides a prospective method for the more effective application of insecticides for the control of in the field.
烟粉虱（Gennadius）是中国最重要的入侵物种之一，具有很强的抗药性和耐热性。在本研究中，我们调查了高温对烟粉虱MEDA1对阿维菌素（AB）和噻虫嗪（TH）杀虫剂耐受性的影响。我们首先从烟粉虱MEDA1中克隆了两个新的CYP450基因，包括一个CYP6家族基因（）和一个CYP305家族基因（）。比较了这两个BtCYP450基因在高温胁迫和接触杀虫剂后的表达模式，然后利用RNA干扰技术来证明这两个基因在杀虫剂耐受性中所起的作用。结果表明，暴露于高温或杀虫剂中可诱导这两个BtCYP450基因的表达，但当昆虫暴露于高温和杀虫剂处理的联合作用时，这种基因表达会受到一定程度的抑制。对于AB处理，当昆虫暴露于41℃并接受AB处理（温度和杀虫剂的联合作用）时，这两个BtCYP450基因的表达达到最低水平。相比之下，TH处理显示随着暴露于高温下，这两个BtCYP450基因的表达普遍下降。这些发现表明，烟粉虱MEDA1的杀虫剂耐受性可能由高温介导。本研究为在田间更有效地应用杀虫剂防治烟粉虱提供了一种前瞻性方法。
