抗嗜肺军团菌血清1型单克隆抗体的制备。

Production of monoclonal antibodies against Legionella pneumophila serogroup 1.

作者信息

Miyazaki T, Koga H, Nakashima M, Tomonaga A, Kohno S, Hirota M, Saito A, Hara K, Watanabe T

出版信息

Microbiol Immunol. 1985;29(3):275-84. doi: 10.1111/j.1348-0421.1985.tb00826.x.

DOI:10.1111/j.1348-0421.1985.tb00826.x

Abstract

Four monoclonal antibodies to Legionella pneumophila Philadelphia 1 were produced by the fusion of immunized BALB/c lymphocytes to a murine myeloma cell line. Two (Lp1-1 and Lp1-3) of the four monoclonal antibodies reacted with 14 L. pneumophila serogroup 1 strains, and the other (Lp1-2 and Lp1-4) reacted with only three out of 20 strains tested. These four monoclonal antibodies did not bind to any strains of L. pneumophila serogroup 2-7 and other Legionella species. In addition, it has been shown that these monoclonal antibodies may be useful not only for subserotyping of L. pneumophila but also for retrospective diagnosis using immunopathological methods.

摘要

通过将免疫的BALB/c淋巴细胞与鼠骨髓瘤细胞系融合，制备了四种抗嗜肺军团菌费城1型的单克隆抗体。四种单克隆抗体中的两种（Lp1-1和Lp1-3）与14株嗜肺军团菌血清1型菌株发生反应，另外两种（Lp1-2和Lp1-4）仅与所检测的20株菌株中的三株发生反应。这四种单克隆抗体不与嗜肺军团菌血清2 - 7型的任何菌株以及其他军团菌属物种结合。此外，已表明这些单克隆抗体不仅可用于嗜肺军团菌的亚血清分型，还可用于采用免疫病理学方法的回顾性诊断。