Radiology and Medical Imaging Department, College of Applied Medical Sciences, Prince Sattam Bin Abdul-Aziz University, Al-Kharj, Saudi Arabia.
Biophysics Department, Faculty of Science, Cairo University, Cairo, Egypt.
J Biomater Sci Polym Ed. 2024 Oct;35(14):2204-2219. doi: 10.1080/09205063.2024.2370591. Epub 2024 Jun 26.
This study aimed to synthesize and characterize chitosan-coated noisomal doxorubicin for the purpose of enhancing its medical application, particularly in the field of cancer treatment. Doxorubicin, a potent chemotherapeutic agent, was encapsulated within noisomes, which are lipid-based nanocarriers known for their ability to efficiently deliver drugs to target sites. Chitosan, a biocompatible and biodegradable polysaccharide, was used to coat the surface of the noisomes to improve their stability and enhance drug release properties. The synthesized chitosan-coated noisomal doxorubicin was subjected to various characterization techniques to evaluate its physicochemical properties. Transmission electron microscopy (TEM) revealed a spherical structure with a diameter of 500-550 ± 5.45 nm and zeta potential of +11 ± 0.13 mV with no aggregation or agglomeration. Chitosan-coated noisomes can loaded doxorubicin with entrapping efficacy 75.19 ± 1.45%. While scanning electron microscopy (SEM) revealed well-defined pores within a fibrous surface. It is observed that chitosan-coated niosomes loading doxorubicin have optimum roughness (22.88 ± 0.71 nm). UV spectroscopy was employed to assess the drug encapsulation efficiency and release profile. Differential scanning calorimetry (DSC) helped determine the thermal behavior, which indicated a broad endotherm peak at 52.4 °C, while X-ray diffraction (XRD) analysis provided information about the crystallinity of the formulation with an intense peak at 23.79°. Fourier-transform infrared spectroscopy (FTIR) indicated the formation of new bonds between the drug and the polymer. The findings from this study will contribute to the knowledge of the physical and chemical properties of the synthesized formulation, which is crucial for ensuring its stability, drug release kinetics, and biological activity. The enhanced chitosan-coated noisomal doxorubicin has the potential to improve the effectiveness and safety of doxorubicin in cancer treatment, offering a promising strategy for enhanced medical applications.
本研究旨在合成并表征壳聚糖包覆的阿霉素纳米囊泡，以提高其在医学领域的应用，特别是在癌症治疗方面的应用。阿霉素是一种有效的化疗药物，被包裹在纳米囊泡中，纳米囊泡是一种基于脂质的纳米载体，以其将药物高效递送至靶部位的能力而闻名。壳聚糖是一种生物相容性和可生物降解的多糖，用于包覆纳米囊泡的表面，以提高其稳定性和增强药物释放性能。所合成的壳聚糖包覆的阿霉素纳米囊泡经过各种表征技术进行评估，以考察其物理化学性质。透射电子显微镜（TEM）显示，球形结构的直径为 500-550±5.45nm，zeta 电位为+11±0.13mV，无聚集或团聚现象。壳聚糖包覆的纳米囊泡可以包载阿霉素，包封率为 75.19±1.45%。扫描电子显微镜（SEM）显示，纤维状表面内有明显的孔。可以观察到，壳聚糖包覆的载药纳米囊泡具有最佳的粗糙度（22.88±0.71nm）。紫外分光光度法用于评估药物包封效率和释放曲线。差示扫描量热法（DSC）有助于确定热行为，表明在 52.4°C 处有一个宽的吸热峰，而 X 射线衍射（XRD）分析提供了关于制剂结晶度的信息，在 23.79°处有一个强峰。傅里叶变换红外光谱（FTIR）表明药物与聚合物之间形成了新的键。这项研究的结果将有助于了解合成制剂的物理和化学性质，这对于确保其稳定性、药物释放动力学和生物活性至关重要。增强型壳聚糖包覆的阿霉素纳米囊泡有可能提高阿霉素在癌症治疗中的有效性和安全性，为增强医学应用提供了一种有前途的策略。
