Mohamad Ebtesam A, Gad Amany M, Abd El-Rhman Rana H, Moselhey Manal T H, Madian Noha G
Radiology and Medical Imaging Department, College of Applied Medical Sciences, Prince Sattam Bin Abdul-Aziz University, 11942, Al-Kharj, Saudi Arabia.
Biophysics Department, Faculty of Science, Cairo University, Giza, Egypt.
Naunyn Schmiedebergs Arch Pharmacol. 2024 Dec 20. doi: 10.1007/s00210-024-03716-1.
Chitosan (CS) has excellent film-forming properties; unfortunately, its use as a film wound dressing is limited because of its weak mechanical properties, especially in its wet state. For this reason, modifications with different materials are investigated in this study. The aim of this work was the combination of chitosan with poly (vinyl alcohol) (PVA), magnesium oxide nanoparticles (MgO), and glycerol as a plasticizer agent which can strengthen CS films, increase their flexibility, and enhance their resistance to microbes. Four types of films were prepared, i.e., CS, PVA, CS/PVA, and CS/PVA@MgO, using solvent casting method. The films' ability for wound dressing was assessed by UV spectroscopy, mechanical properties, Fourier transform infrared, X-ray diffraction, antimicrobial activity, and in vivo studies as a practical application on wounds. CS/PVA@MgO showed an improvement in mechanical properties as it has elongation at break of 522% and strain 585%. In addition, the antimicrobial activity of CS/PVA@MgO film was extensively enhanced as it inhibited Escherichia coli, Staphylococcus aureus, and Candida albicans by 90.78%, 88.83%, and 97.18%, respectively. The results showed that composite film has a good mechanical properties and antimicrobial activity expressed a suitable wound dressing material. Furthermore, in vivo experiment evaluated the clinical efficacy of CS/PVA@MgO film in wound healing.
壳聚糖（CS）具有优异的成膜性能；然而，由于其机械性能较弱，尤其是在湿态下，其作为薄膜伤口敷料的应用受到限制。因此，本研究对其与不同材料的改性进行了探究。这项工作的目的是将壳聚糖与聚乙烯醇（PVA）、氧化镁纳米颗粒（MgO）以及作为增塑剂的甘油相结合，以增强CS薄膜的性能，提高其柔韧性，并增强其抗菌能力。采用溶液浇铸法制备了四种类型的薄膜，即CS、PVA、CS/PVA和CS/PVA@MgO。通过紫外光谱、机械性能、傅里叶变换红外光谱、X射线衍射、抗菌活性以及作为伤口实际应用的体内研究来评估这些薄膜作为伤口敷料的能力。CS/PVA@MgO的机械性能有所改善，其断裂伸长率为522%，应变率为585%。此外，CS/PVA@MgO薄膜的抗菌活性显著增强，它对大肠杆菌、金黄色葡萄球菌和白色念珠菌的抑制率分别为90.78%、88.83%和97.18%。结果表明，复合薄膜具有良好的机械性能和抗菌活性，是一种合适的伤口敷料材料。此外，体内实验评估了CS/PVA@MgO薄膜在伤口愈合方面的临床疗效。
